物理化学学报 >> 1991, Vol. 7 >> Issue (04): 490 -494 .doi: 10.3866/PKU.WHXB19910422

研究简报 上一篇    下一篇

用微量热法研究漆酶和过氧化氢的反应

吴鼎泉; 梅付明; 屈松生; 杜予民; 詹东风   

  1. 武汉大学化学系,武汉 430072;武汉大学资源化学研究所
  • 收稿日期:1990-03-07 修回日期:1991-02-02 发布日期:1991-08-15
  • 通讯作者: 吴鼎泉

Study on the Reaction of Laccase and Hydrogen Peroxide by Microcalorimetry

Wu Ding-Quan; Mei Fu-Ming; Qu Song-Sheng; Du Yu-Min; Zhan Dong-Feng   

  1. Department of Chemistry, Wuhan University, Wuhan 430072; Institute of Resource Chemistry, Wuhan University
  • Received:1990-03-07 Revised:1991-02-02 Published:1991-08-15
  • Contact: Wu Ding-Quan

关键词: 微量热法, 速度常数, 来氏常数, 漆酶, 底物

Abstract: Study on thermokinetic properties of the reaction between laccase and hydrogen peroxide by microcalorimetry has been carried out. The rate constant of the reaction is k_1=4.15×10~(-4) s~(-1). The reoxidation of the product of the reaction of laccase with hydroquinone by hydrogen peroxide obeys Michaelis-Menten's mechanism. The Michael constant K_m equals 0.0392M.

Key words: Microcalorimetry, Rate constant, Michaelis constant, Laccase, Substrate