物理化学学报 >> 1992, Vol. 8 >> Issue (04): 493-499.doi: 10.3866/PKU.WHXB19920413

研究论文 上一篇    下一篇

平菇多糖稀溶液行为的研究

郑昌仁; 朱志敏; 谷云乐; 胡雷; 傅庭治; 欧惠春   

  1. 南京化工学院化工系,南京 210009;南京大学生物系
  • 收稿日期:1991-03-25 修回日期:1991-11-26 发布日期:1992-08-15
  • 通讯作者: 郑昌仁

Studies on the Dilute Solution Behavior of Polysaccharide of Pleuratu Ostretus

Zheng Chang-Ren; Zhu Zhi-Min; Gu Yun-Le; Hu Lei; Fu Ting-Zhi; Ou Hui-Chun   

  1. Nanjing Institute of Chemical Technology, Nanjing 210009; Department of Biology, Nanjing University
  • Received:1991-03-25 Revised:1991-11-26 Published:1992-08-15
  • Contact: Zheng Chang-Ren

摘要: 平菇多糖属生物高分子, 文章对平菇多糖稀溶液性质进行了研究。实验系采用Elias浊点滴定法测定平菇多糖溶液的θ组成, 用快速动态膜渗透计和粘度法测定平菇多糖的数均分子量、维利系数与分子链的构型参数。探讨其在θ条件下的分子形态及用Tung函数法等处理分级数据, 从而获得平菇多糖的分子量分布。

关键词: 多糖, 稀溶液, 平菇, 分子量, 浊度滴定

Abstract: The polysaccharide of pleuratu ostreatus was fractionated by repeated precipitation method. Modified Elias cloud-point titration method with an UV detecter was used to determine the cloud-point. It was found that water/acetone (83.5:16.5 by volume) is a θ-solvent for the polymer at 25 ℃. Number average molecular weights of all samples fractionated were characterized by rapid dynamic membrane osmometry and viscometry. The second virial coefficient A_2 decreases as the molecular weight in-creases. The relationship is A_2=2 slope (M_nRT)~(-1/2).
The intrinsic viscosities were determined by capillary viscometry in θ-solvent. Thus and thus a mondispersed [η]~M relationship has been obtained:
[η]_θ=0.124~(0.513)
The unperturbed dimension ...

Key words: Pleuratu ostreatus, Polysaccharide, Dilute solution, Molecular weight, Cloud-point titration