物理化学学报 >> 1994, Vol. 10 >> Issue (03): 266-275.doi: 10.3866/PKU.WHXB19940314

研究论文 上一篇    下一篇

二甲基亚砜及一些含氮环化合物与DNA的作用

王保怀, 张开江, 李芝芬, 张有民, 杨铭, 王夔   

  1. 北京大学物理化学研究所,北京 100871|北京医科大学天然及仿生药物国家重点实验室,北京 100083
  • 收稿日期:1992-12-09 修回日期:1993-05-16 发布日期:1994-03-15
  • 通讯作者: 王保怀

Effect of Dimethyl Sulphoxide and Some Multicycles Compounds with Nitrogen Atoms on Calf Thymus DNA

Wang Bao-Huai, Zhang Kai-Jiang, LI  Zhi-Fen, Zhang You-Min, Yang Ming, Wang Kui   

  1. Institute of Physical Chemistry, Peking University, Beijing 100871| Research Laboratory of Natural and Biomimetic Drugs, Beijing Medical University, Beijing 100083
  • Received:1992-12-09 Revised:1993-05-16 Published:1994-03-15
  • Contact: Wang Bao-Huai

摘要:

通过实验探索了将既不溶于水又不能生成可溶性盐的一些含氮稠环化合物溶于二甲基亚砜(DMSO)水溶液中与小牛胸腺DNA作用的途径.溶解实验证明了DMSO及其水溶液对此类化合物极强的溶解性能,DMSO与小牛胸腺作用的紫外光谱(298 K)、圆二色谱(298 K)、~(31)P核磁谱(323 K)和摩尔焓变(298 K)数据均表明:在pH=7.0的水溶液中,当DMSO浓度小于0.15 mol·L~(-1)时,其与DNA无作用.溶于DMsO水溶液中的八种含氮稠环化合物与小牛胸腺DNA作用的紫外光谱(298 K)、圆二色谱(298 K)和摩尔焓变(298 K)实验结果显示出两种化合物对DNA有较强的嵌插作用.通过对实验结果的分析,本文对具有相同含氮稠环骨架的化合物与DNA嵌播作用的规律和化合物本身微观结构的关系进行了讨论.

关键词: 二甲基亚砜, 稠环化合物, 选择溶剂化, 嵌插作用

Abstract:

A method for studying the effect of some organic compounds, that are neither soluble in water nor to be made into soluble salts in water on DNA has been investigated by a series of experiments. The solubility experiments have shown that dimethyl sulphoxide(DMSO) and its aqueous solutions are excellent solvents for the hydrophobic multicycles compounds with nitrogen atoms. The data of UV(298 K), CD(298 K), ~(31)P-NMR(323 K) spectra and molar enthalpy changes (298 K) for the effect of DMSO on DNA have not shown any effect of DMSO on DNA when the pH of solution is equal 7.0 and its concentration is less than 0.15 mol·L~(-1). The results of UV (298 K), CD(298 K) spectra and molar enthalpy changes (298 K) for the effect of eight multicycles compounds with nitrogen atoms on DNA in aqeous solution of DMSO have indicated that two of them have strong intercalation binding to DNA. Both the mechanism of interaction between the above compounds and DNA and the relationship between the behavior of intercalation binding of the compounds with same multicycles structures to DNA and their microstructures have been discussed.

Key words: Dimethyl sulphoxide, Multicycles compounds, Selective solvation, Intercalation binding