物理化学学报 >> 1995, Vol. 11 >> Issue (12): 1077-1083.doi: 10.3866/PKU.WHXB19951204

研究论文 上一篇    下一篇

维生素E与磷脂酰胆碱的单分子膜研究

莫凤奎,王晶,植松正吾,赤土屈幸男   

  1. 沈阳药科大学基础部,沈阳 110015|静冈县立大学药学部,静冈市,日本国 422
  • 收稿日期:1995-01-16 修回日期:1995-05-09 发布日期:1995-12-15
  • 通讯作者: 莫凤奎

Study on Monolayer of Vitamin E and Phosphatidylcholines

Mo Feng-Kui,Wang Jing,Shogo Uematsu,Yukio Akahori   

  1. Department of Basic Courses,Shenyang Pharmaceutical University,Shenyang 110015|Department of Pharmaceutical Science,University of Shizuoka,Shizuoka,Japan 422
  • Received:1995-01-16 Revised:1995-05-09 Published:1995-12-15
  • Contact: Mo Feng-Kui

摘要:

测定了下列气水界面单分子膜的表面压-平均分子面积等温线:(1)d-α-;d-β-,d-γ-,和d-δ-生育酚等4种维生素E与DPPC,DOPC及DLPC的混合物;(2)d-α-生育酚等摩尔比的DPPC和DLPC的混合物;(3)胆固醇与DPPC,DLPC的混合物. 讨论了维生素E色满环上甲基对其在PC单分子膜中物理化学作用的影响. 实验结果表明:(1)维生素E以d-α->d-β≈d-γ->d-δ-的次序引起DPPC单分子膜的膨胀,以相反的次序使DOPC单分子膜凝缩。(2)维生素E可降低DPPC单分子膜的崩裂压,但不引起DOPC和DLPC单分子膜崩裂压的改变。(3)向等摩尔比的DOPC和DLPC的混合单分子膜中混入生育酚不改变膜的崩裂压,但引起膜的膨胀。(4)与一般认为生育酚和胆固醇在磷脂膜中的物理化学作用彼此类似的看法不同,它们有着明显的差别

关键词: 单分子膜, 维生素E, 磷脂酰胆碱, 生育酚, 胆固醇, 表面压

Abstract:

The surface pressure-area isotherms of mixed monolayers at the air/water interface have been determined for systems: d-α-, d-β-, d-γ-, and d-δ-tocopherol(TOC) with dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine(DOPC); d-α-TOC and cholesterol (CH) with dilinoleoylphosphatidylcholine (DLPC);and d-α-TOC with DPPC/DLPC (1:1/mol:mol). The effect of the chromanol methyl groups of vitamin E(VE) on its physicochemical behaviors in these monolayers had been discussed. It was observed that (1) TOC caused the DPPC monolayer to expand in the order ofα>β≈γ>δ, and the DOPC monolayer to condense in reverse order; (2) The collapse pressure of monolayer for DOPC and DLPC was not changed by TOC, while that of DPPC decreased; (3) Addition of d-α-TOC into the monolayer of equimolar mixture of DPPC and DLPC did not change the collapse pressure but produced an expanding effect; (4) Contrary to the generally accepted view, the physicochemical function of VE in mixed monolayer of phospholipids was obviously different from that of CH.

Key words: Monolayer, Vitamin E, Phosphatidylcholine, Tocopherol, Cholesterol, Surfacerol, Surface pressure