物理化学学报 >> 1996, Vol. 12 >> Issue (04): 310-314.doi: 10.3866/PKU.WHXB19960405

研究论文 上一篇    下一篇

别嘌呤醇对黄嘌呤氧化酶的抑制作用研究

薛怀国,仲雷,邵亮,阚锦晴,穆绍林   

  1. 扬州大学师范学院化学系,扬州 225002
  • 收稿日期:1995-06-30 修回日期:1995-09-29 发布日期:1996-04-15
  • 通讯作者: 穆绍林

Stusy on Inhibition of Xanthine Oxidase by Allopurinol

Xue Huai-Guo,Zhong Lei,Shao Liang,Kan Jin-Qing,Mu Shao-Lin   

  1. Department of Chemistry,Teacher's College,Yangzhou University,Yangzhou 225002
  • Received:1995-06-30 Revised:1995-09-29 Published:1996-04-15
  • Contact: Mu Shao-Lin

摘要:

用聚苯胺黄嘌呤氧化酶电极(生物传感器)研究了别嘌呤醇对黄嘌呤氧化酶的抑制作用. 实验结果表明:别嘌呤醇对黄嘌呤氧化酶有明显的抑制作用,但这种抑制作用是可逆的.抑制剂的存在使固定酶的表观米氏常数增大,但并没有影响酶催化反应的最大速率.所以别嘌呤醇是黄嘌呤氧化酶的一种可逆竞争抑制剂. 抑制剂对固定酶的最适pH值没有影响,而对反应活化能略有影响。

关键词: 生物传感器, 黄嘌呤氧化酶, 抑制作用

Abstract:

The investigation of inhibition of xanthine oxidase by allopurinol was carried out using a xanthine biosensor, which was obtained using the polyaniline film as a support material. The experimental results indicated that significant inhibition could be produced by allopurinol but this inhibition was reversible. The apprent Michaelis-Menten constant (K’m) of the immobilized xanthine oxidase increased in the presence of allopurinol, but the maximum rate of the enzyme catalyzed reaction was not affected by allopurinol. This indicated that allopurinol is a reversible competitive inhibitor of xanthine oxidase. The optimum pH of the immobilized enzyme was not affected, and the activation energy of the enzyme catalyzed reaction was slightly affected by allopurinol.

 

Key words: Biosensor, Xanthine oxidase, Inhibition