物理化学学报 >> 1996, Vol. 12 >> Issue (06): 543-546.doi: 10.3866/PKU.WHXB19960613

研究简报 上一篇    下一篇

两种水溶性卟啉与DNA相互作用的研究

熊亚,黄素秋,吴鼎泉,屈松生   

  1. 武汉大学化学系,武汉 430072
  • 收稿日期:1995-09-13 修回日期:1995-12-14 发布日期:1996-06-15
  • 通讯作者: 屈松生

Studies on the Interactions of Two Kinds of Water-Soluble Porphyrins with DNA

Xiong Ya,Huang Su-Qiu,Wu Ding-Quan,Qu Song-Sheng   

  1. Department of Chemistry,Wuhan University,Wuhan 430072
  • Received:1995-09-13 Revised:1995-12-14 Published:1996-06-15
  • Contact: Qu Song-Sheng

关键词: 卟啉, DNA, 微量热法, 紫外光谱, 相互作用

Abstract:

The interactions of two kinds of water-soluble porphyrins, Cu (NEAE) and H2PPS, with DNA have been investigated by microcalorimetry and UV spectroscopy. The experimental results show that Cu (NEAE) interact with dsDNA in the outside-bound mode, its binding number is 9~10 base pairs and its binding enthalpy △H=-24.4kJ.mol-1. There are large enthalpy changes when Cu (NEAE) react with ssDNA. H2PPS can not react with both dsDNA and ssDAN, which demonstrats that the coulombic force is one of the important factors affecting the interactions of porphyrins with DNA.

Key words: Porphyrin, DNA, Microcalorimetry, UV spectroscopy, Interaction