物理化学学报 >> 1997, Vol. 13 >> Issue (11): 969-973.doi: 10.3866/PKU.WHXB19971103

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微管蛋白聚合及其与紫杉醇作用的量热学研究

韩公社,阎隆飞,王保怀,张有民   

  1. 中国农业大学生物学院，北京 100094|北京大学物理化学研究所，北京 100871
  • 收稿日期:1997-08-25 修回日期:1997-09-22 发布日期:1997-11-15
  • 通讯作者: 王保怀

Microcalorimetric Studies of the Tubulin Polymerization and the Interaxtion between Tubuin and Taxol

Han Gong-She,Yan Long-Fei,Wang Bao-Huai,Zhang You-Min   

  1. College of Biological Science,China Agricultural University,Beijing 100094|Department of Chemistry,Peking University,Beijing 100871
  • Received:1997-08-25 Revised:1997-09-22 Published:1997-11-15
  • Contact: Wang Bao-Huai

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关键词: 微管蛋白, 紫杉醇, 热谱图

Abstract:

The heat properties of porcine brain tubulin polymerization and the interaction of the tubulin with taxol were studied with an MS-80 Calvet Microcalorimeter .The heat vibration occured within 1.5h when tubulin solution was diluted from a highe r concentration to a lower one. The thermodynamic parameters of tubulin polymerization at 303.15K and 298. 15K were measured in 10μmol•L-1 tubulin solution, its △H's were 22kJ•mol-1 and 29.3kJ•mol-1，respectively.Adding different concentration of taxol in the course of polymerization of tubulin give rise to a great change in △H. The time of taxol combining to polymerized microtubule was about one minute after the beginning of the reaction. The mechanism of tubulin polymerization under different conditions was discussed.

Key words: Tubulin, Taxol, Thermogram