物理化学学报 >> 1997, Vol. 13 >> Issue (11): 969-973.doi: 10.3866/PKU.WHXB19971103
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韩公社,阎隆飞,王保怀,张有民
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Han Gong-She,Yan Long-Fei,Wang Bao-Huai,Zhang You-Min
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关键词: 微管蛋白, 紫杉醇, 热谱图
Abstract:
The heat properties of porcine brain tubulin polymerization and the interaction of the tubulin with taxol were studied with an MS-80 Calvet Microcalorimeter .The heat vibration occured within 1.5h when tubulin solution was diluted from a highe r concentration to a lower one. The thermodynamic parameters of tubulin polymerization at 303.15K and 298. 15K were measured in 10μmol•L-1 tubulin solution, its △H's were 22kJ•mol-1 and 29.3kJ•mol-1,respectively.Adding different concentration of taxol in the course of polymerization of tubulin give rise to a great change in △H. The time of taxol combining to polymerized microtubule was about one minute after the beginning of the reaction. The mechanism of tubulin polymerization under different conditions was discussed.
Key words: Tubulin, Taxol, Thermogram
韩公社,阎隆飞,王保怀,张有民. 微管蛋白聚合及其与紫杉醇作用的量热学研究[J]. 物理化学学报, 1997, 13(11): 969-973.
Han Gong-She,Yan Long-Fei,Wang Bao-Huai,Zhang You-Min. Microcalorimetric Studies of the Tubulin Polymerization and the Interaxtion between Tubuin and Taxol[J]. Acta Phys. -Chim. Sin., 1997, 13(11): 969-973.
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http://www.whxb.pku.edu.cn/CN/Y1997/V13/I11/969
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