物理化学学报 >> 1998, Vol. 14 >> Issue (07): 649-653.doi: 10.3866/PKU.WHXB19980715

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BSA和SDBS在水溶液与空气界面的相互作用

毕只初, Neuman A W   

  1. 中国科学院感光化学研究所，北京 100101|Laboratory of Applied Surface Thermodynamics,University of Toronto,Canada
  • 收稿日期:1997-09-15 修回日期:1997-12-22 发布日期:1998-07-15
  • 通讯作者: 毕只初

Interaction between BSA and SDBS at Water-air Interface by ADSA Method

Bi Zhi-Chu, Neuman A W   

  1. Institute of photographic Chemistry,Chinese Academy of Sciences,Beijing 100101|Laboratory of Applied Surface Thermodynamics,University of Toronto,Canada
  • Received:1997-09-15 Revised:1997-12-22 Published:1998-07-15
  • Contact: Bi Zhi-Chu

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关键词: 液滴形状分析, 数字影像处理, 动态表面张力, 蛋白质, 相互作用

Abstract:

The interaction between BSA (Bovine Serium Albumin)and SDBS (Sodium Dodecyl Benzene sulfonate) at water - air interface of aqueous solution was investigated by ADSA(Axisymmetric Drop Shape Analysis) method. The surface tension responses to time were measured and compared. The results showed that (1) ADSA is a good methed to study the dynamic change of surface tension to the time; (2) While SDBS concentration was less than its CMC, it could interact with BSA macro-molecule in the solution to form a complex which was more surface active than both BSA and SDBS, and its conformation change in the adsorption layer was similar with that of BSA alone, (3) While SDBS concentration was larger than its CMC, its micelle could solubilize the BSA macro-molecule and the surface tension and the conformation change in the adsorption layer was very similar with these of SDBS solution alone.

Key words: Drop shape analysis, Digital image process, Dynamic surface tersion, Protein, Interaction