物理化学学报 >> 2000, Vol. 16 >> Issue (03): 232-237.doi: 10.3866/PKU.WHXB20000308

研究论文 上一篇    下一篇

环-Phe-His二肽水溶液的激光光解

储高升, 宋钦华, 王忠义, 葛学武, 张志成, 王文锋, 姚思德   

  1. 中国科学技术大学应用化学系,化学系,合肥 230026|中国科学院上海原子核研究所辐射化学开放研究实验室,上海 201800
  • 收稿日期:1999-06-04 修回日期:1999-10-08 发布日期:2000-03-15
  • 通讯作者: 储高升

Laser Flsah Photolysis Study of Cyclic-Phe-His in Aqueous Solution

Chu Gao-Sheng, Song Qin-Hua, Wang Zhong-Yi, Ge Xue-Wu, Zhang Zhi-Cheng, Wang Wen-Feng, Yao Si-De   

  1. Department of Applied Chemistry,Department of Chemistry,University of Science and Technology of China,Hefei 230026|Laboratory of Radiation Chemistry,Shanghai Institute of Nuclear Research,Chinese Academy of Sciences,Shanghai 201800
  • Received:1999-06-04 Revised:1999-10-08 Published:2000-03-15
  • Contact: Chu Gao-Sheng

摘要:

报导了利用纳秒级激光光解瞬态吸收光谱技术研究环苯丙氨酰组氨酰水溶液光解和光敏化作用过程. 发现了该环肽在248nm激光的激励下产生光电离和光解离, 光电离和光解离过程发生在苯丙氨酸残基上,生成具有320nm和410nm特征吸收的自由基,光解离产生具有320nm特征吸收的苄基自由基. 而具有亲电子性的丙酮能在该环肽的苯丙氨酸残基的苯环上抽取电子,形成320nm和410nm的特征吸收峰,求出了自由基的有关动力学参数,并用脉冲辐解动态吸收光谱法印证了上述结果.

关键词: 环苯丙氨酰组氨酰, 光电离, 光解离, 激光光解, 脉冲辐解

Abstract:

Photolysis and photosensition of cyclic-phenylalanine-histidine(cyclic-Phe-His) in aqueous solution were studied using KrF laser flash photolysis (248nm). Photoionization and photodissociation of cyclic-Phe-His in Phe-site occured and the charateristic absorption at 320nm and 410nm have been observed. From changes of the absorption peaks and kinetic analysis, the reaction mechanism of photoionization and photodissociation has been suggested, a series of kinetic parameters have been obtained. Using acetone as a photosensitizer, and N3• as an oxidant by pulse radiolysis method, the induced oxidation of cyclic-Phe-His in aqueous solution has been also reported in this paper, results similar to the photoionization of cyclic-Phe-His have been found.

Key words: Cyclic-phenylalanyl-histidyl(cyclic-Phe-His), Photoionization, Photodissociation, Laser flash photolysis, Pulse radiolysis