物理化学学报 >> 2000, Vol. 16 >> Issue (05): 398-401.doi: 10.3866/PKU.WHXB20000504
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姜凌, 毛希安
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Jiang Ling, Mao Xi-an
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关键词: 核苷酸, 碱基堆积, 二维NOE谱(NOESY)
Abstract:
The concept of base stacking of nucleotides in solutions has been well accepted. However, direct evidence has not been found. Here we report for the first time the intermolecular NOE cross peaks between ATP (adenosine 5’-triphosphate) and GTP (guanosine 5’-triphosphate), which unambiguously indicates the base stacking of nucleotides in solutions. In 400 mmol•L -1 ATPGTP solutions, the base protons of ATP, AH2 and AH8, show NOE cross peaks with the sugar protons of GTP, GH1′,GH2′ and GH3′.Also there are NOE cross peaks between the base proton of GTP (GH8) and the sugar protons of ATP. Possible conformations of base stacking in solutions have been discussed, and the distance between the stacked bases is estimated.
Key words: Nucleotide, Base stacking, NOESY
姜凌, 毛希安. 核苷酸碱基堆积导致的分子间NOE交叉峰[J]. 物理化学学报, 2000, 16(05): 398-401.
Jiang Ling, Mao Xi-an. Base-Stacking-Induced Intermolecular NOE Cross Peaks for Nucleotides[J]. Acta Phys. -Chim. Sin., 2000, 16(05): 398-401.
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链接本文: http://www.whxb.pku.edu.cn/CN/10.3866/PKU.WHXB20000504
http://www.whxb.pku.edu.cn/CN/Y2000/V16/I05/398
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