物理化学学报 >> 2000, Vol. 16 >> Issue (08): 729-734.doi: 10.3866/PKU.WHXB20000811

研究论文 上一篇    下一篇

环方铂立体异构体与小牛胸腺DNA作用的研究

周力, 王保怀, 李芝芬, 李册, 杨铭   

  1. 北京大学物理化学研究所，北京 100871|北京联合大学应用文理学院，北京100083|北京医科大学 天然及仿生药物国家重点实验室，北京 100083
  • 收稿日期:1999-10-29 修回日期:2000-03-22 发布日期:2000-08-15
  • 通讯作者: 王保怀

Interactions of Dioxycyclobutenedione-(1,2-cyclohexanediamine) Platinum(II) with Calf Thymus SNA

Zhou Li, Wang Bao-Huai, Li Zhi-Fen, Li Ce, Yang Ming   

  1. Institute of Physical Chemistry,Peking University,Beijing 100871|College of Applied Sciences and Humanities of Beijing United University,Beijing 100083|Research Laboratoriy of Natural and Biomimetic Drugs,Beijing Medical University,Beijing 100083
  • Received:1999-10-29 Revised:2000-03-22 Published:2000-08-15
  • Contact: Wang Bao-Huai

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摘要：

研究了环方铂三种立体异构体（ＳＳ，ＲＳ和ＲＲ）与小牛胸腺ＤＮＡ的作用，差示扫描量热法（ＤＳＣ）测得的数据显示出三种化合物与ＤＮＡ作用的结果分别使其熔融温度下降了０.６，１.４和２.２Ｋ．有无ＤＮＡ存在时三种化合物及方酸（ｐＨ＝７.０）的１３Ｃ核磁共振谱表明，当三种化合物与ＤＮＡ作用时，先释放出方酸根生成水铂，而后再与ＤＮＡ结合．综合上述实验结果，对三种化合物与ＤＮＡ的作用强度、方式及机理进行了讨论．

关键词: 环方铂, 小牛胸腺DNA, DSC, NMR spectra

Abstract:

The interaction of a group of new synthetic compounds, dioxycyclobutenedione- (1, 2 -cyclohexanedimine ) platinum (Ⅱ) (DCP ) and its stereoisomers, with calf thymus DNA was investigated by DSC and NMR methods. In the presence of three stereoisomers of the compounds, the melting temperatures of DNA are 350.45 (SS, trans-d-l-DCP), 349.65 (RS, cis-DCP) and 348.85(RR, trans-1-DCP) )K respectively, while the melting temperature of DNA is 351.05 K. Comparing the 13C NMR spectra of three stereoisomers of the compounds with and without DNA to that of squaric acid at pH = 7. 0 and room temperature, it can be obviously seen that square acid bases dropped out of the compound molecules as leaving group and got into the solutions as they bound to DNA. On the basis of the above experimental results, the binding strength and the model of the compounds with DNA have been discussed.

Key words: Dioxycyclobutenedione-(1,2-cyclohexanediamine) platinum(II), Calf thymus DNA, DSC, NMR spectra