物理化学学报 >> 2000, Vol. 16 >> Issue (09): 830-834.doi: 10.3866/PKU.WHXB20000913

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聚苯胺用作乙醇脱氢反应中的电子传递介质

穆绍林, 杨一飞   

  1. 扬州大学理学院化学系,扬州 225002
  • 收稿日期:1999-11-23 修回日期:2000-02-08 发布日期:2000-09-15
  • 通讯作者: 穆绍林

Polyaniline Used as An Electron Transfer Mediator for the Catalyzed Dehydrogenating Renction of Alcohol

Mu Shao-Lin, Yang Yi-Fei   

  1. Department of Chemistry,School of Sciences,Yangzhou University,Yangzhou 225002
  • Received:1999-11-23 Revised:2000-02-08 Published:2000-09-15
  • Contact: Mu Shao-Lin

关键词: 乙醇脱氢酶的固定, 聚苯胺, 电子传递介质, 乙醇脱氢反应

Abstract:

The aniline solution containing nicotinamide adenine dinucleotide(NAD+ )was electrolyzed. Polyaniline film with NAD+ formed on the platinum thin plate. The thickness of the film was controlled by the charge consumed in the electrolysis, which was 0.006 C in this case. This electrode, used as an anode, was immersed in the solution containing aniline. A second film formed on this electrode during the electrolysis. The charge passed during the second electrolysis was 0.016 C. Alcohol dehydrogenase(ADH) was immobilized on the polyaniline film based on the isoelectric point of ADH and the doping principle of conducting polymers. The enzyme electrode has a good bioelectrochemical response to ethanol in the absence of a homogeneous mediator. The optimum pH, apparent Michaelis- Menten constant and optimum temperature of ADH immobilized in polyaniline film are 7.83, 7.5 mmol• dm- 3, and 30.6 ℃ , respectively. The activation energy of the enzyme- catalyzed reaction is 33.5 kJ• mol- 1. The response current of the enzyme electrode increases linearly with increasing concentration of ethanol below 2.4 mmol• dm- 3.This enzyme electrode can be used to determine the concentration of ethanol.

Key words: Immbobilization of alcohol dehydrogenase, Polyaniline, Electron transfer meditor, Dehydrogenating reaction of alcohol