物理化学学报 >> 2001, Vol. 17 >> Issue (01): 48-52.doi: 10.3866/PKU.WHXB20010110

研究论文 上一篇    下一篇

环丙沙星与牛血清白蛋白的结合反应

商志才;易平贵;俞庆森;林瑞森   

  1. 浙江大学化学系, 杭州 310027
  • 收稿日期:2000-05-15 修回日期:2000-07-25 发布日期:2001-01-15
  • 通讯作者: 商志才 E-mail:shangzc@mail.hz.zj.cn

Reaction Mechanism between Ciprofloxacin Hydrochloride and Bovine Serum Albumin

Shang Zhi-Cai;Yi Ping-Gui;Yu Qing-Sen;Lin Rui-Sen   

  1. Department of chemistry,Zhejiang University,Hangzhou 310027
  • Received:2000-05-15 Revised:2000-07-25 Published:2001-01-15
  • Contact: Shang Zhi-Cai E-mail:shangzc@mail.hz.zj.cn

摘要: 以光谱技术与微量热技术相结合研究了水溶液中牛血清白蛋白与环丙沙星分子间结合作用的机制. 荧光猝灭法测得该反应的结合常数K=8.39×104 L•mol-1,结合位点数n =1.18;依据Fōrster非辐射能量转移机制,得到授体受体间的结合距离(r=2.46 nm)和能量转移效率(E=0.33).微量热法测得反应的焓变 ΔrHm≈0;牛血清白蛋白与环丙沙星分子间有较强的结合作用,且以疏水作用为主.

关键词: 环丙沙星, 牛血清白蛋白, 荧光法, 微量热法

Abstract: The reaction between ciprofloxacin and bovine serum albumin(BSA) in aqueous solution was studied by fluorescence spectra and microcalorimetry.The binding constant K was found to be 8.39×104L•mol-1 and the binding sites n to be 1.18 by fluorescence quenching method.The binding distance (r=2.46 nm) and transfer efficiency (E=0.33) between ciprofloxacin and BSA were also obtained according to the Fōrster theory of non-radiation energy transfer.Microcalorimetric measurements showed that the molar change of enthalpy ΔrHm≈0 for the reaction.The interaction between ciprofloxacin and BSA is stronger and the main binding force is hydrophobic interaction.

Key words: Ciprofloxacin, Bovine serum albumin, Fluorescence, Microcalorimetry