物理化学学报 >> 2001, Vol. 17 >> Issue (01): 66-69.doi: 10.3866/PKU.WHXB20010114

研究简报 上一篇    下一篇

沙冬青抗冻蛋白热滞活性的DSC研究

周晓蕾;陈滔滔;王保怀;李芝芬;费云标;魏令波;高素琴   

  1. 北京大学物理化学研究所, 北京 100871;中国科学院发育研究所, 北京 100080
  • 收稿日期:2000-03-14 修回日期:2000-06-08 发布日期:2001-01-15
  • 通讯作者: 王保怀 E-mail:bhwang@pku.edu.cn

DSC Study on the Thermal Hysteresis Activity of Plant Antifreeze Proteins

Zhou Xiao-Lei;Chen Tao-Tao;Wang Bao-Huai;Li Zhi-Fen;Fei Yun-Biao;Wei Ling-Bo;Gao Su-Qin   

  1. Institute of Physical Chemistry, Peking University, Beijing 100871; Institute of Developmental Biology, Chinese Academy of Sciences,Beijing 100080
  • Received:2000-03-14 Revised:2000-06-08 Published:2001-01-15
  • Contact: Wang Bao-Huai E-mail:bhwang@pku.edu.cn

关键词: 抗冻蛋白, DSC, 热滞活性

Abstract: Differential scanning calorimetry (DSC) was used to measure the thermal hysteresis activity(THA) of plant antifreeze proteins(AFPs). The results reveal that DSC is a good method to screen and study AFPs. In the sixteen components extracted from Ammopipanthus mongolicus leaves, one(P3S1) was found to have apparent thermal hysteresis activity by DSC. As the amount of ice nuclei in the sample decreased, the THA of P3S1 increased from 0.01 ℃ to 0.65 ℃. It is notable that the twopeak thermal hysteresis effect was observed. Two endothermic peaks appeared in the melting process of P3S1, while the freezing peak also consisted of two peaks. The peaks appeared antecedently showed larger thermal effect. This phenomenon shows P3S1 has two different kinds of interaction with water and ice crystal. It is probably an important property of a class of AFPs.

Key words: Antifreeze proteins, DSC, Thermal hysteresis activity