物理化学学报 >> 2001, Vol. 17 >> Issue (02): 101-106.doi: 10.3866/PKU.WHXB20010202

研究论文 上一篇    下一篇

水溶性锰卟啉模拟酶体系快速混合停流谱研究

李臻;夏春谷   

  1. 中国科学院兰州化学物理研究所 羰基合成与选择氧化国家重点实验室,兰州 730000
  • 收稿日期:2000-05-16 修回日期:2000-07-17 发布日期:2001-02-15
  • 通讯作者: 夏春谷 E-mail:cyxia@ns.lzb.ac.cn

Rapid-Mixing Stopped-Flow Spectra Studies on Water-soluble Manganese Porphyrin Enzyme-Mimic Systems

Li Zhen;Xia Chun-Gu   

  1. State Key Laboratory of Oxo Synthesis&Selective Oxidation,Lanzhou Institute of Chemical Physics,Chinese Academy of Science,Lanzhou 730000
  • Received:2000-05-16 Revised:2000-07-17 Published:2001-02-15
  • Contact: Xia Chun-Gu E-mail:cyxia@ns.lzb.ac.cn

摘要: 采用快速混合停流技术,考察了不同pH值条件下水溶性锰卟啉MnⅢ(TMPyP)与四种单氧给体mCPBA、KHSO5、NaOCl及H2O2构建的细胞色素P450模拟酶体系催化氧化活性物种的生成及催化烯烃环氧化过程.低pH值时,主要生成四价锰氧中间体2;而在高pH值时,首先生成五价锰氧中间体1,该中间体再向四价中间体2转化.

关键词: 水溶性锰卟啉, 单氧给体, 催化氧化, 停流技术

Abstract: The reaction of watersoluble Manganese porphyrin complex,MnⅢ(TMPyP) with a variety of oxidants(mCPBA,KHSO5,NaOCl and H2O2) under ambient conditions at different pH was investigated by stoppedflow techniques.Oxomanganese(IV) porphyrin complex intermediate (2) was the main product at lower pH.Highvalent oxomanganese(V) porphyrin (1) was fully formed and subsequently decayed to oxoMn(Ⅳ) at higher pH.It was shown that oxomanganese(V) porphyrin was the reactive complex with reducing substrates.

Key words: Water-soluble manganese porphyrin complexes, Single oxygen donors,  Catalytic oxidation, Stopped-flow technique