物理化学学报 >> 2002, Vol. 18 >> Issue (06): 490-494.doi: 10.3866/PKU.WHXB20020603

研究论文 上一篇    下一篇

寡聚脱氧核苷酸吸附状态随电位的变化

沈鹤柏;周文骏;杨海峰;余沛涛   

  1. 上海师范大学生命与环境科学学院化学系, 上海 200234
  • 收稿日期:2001-10-16 修回日期:2002-01-08 发布日期:2002-06-15
  • 通讯作者: 沈鹤柏 E-mail:shenhb@shtu.edu.cn

Change of Adsorption State of Oligodeoxynucleotides on the Silver Electrode Surface with Change of Potential

Shen He-Bai;Zhou Wen-Jun;Yang Hai-Feng;Yu Pei-Tao   

  1. Chemistry Department of Life and Environmental Science College, Shanghai Normal University, Shanghai 200234
  • Received:2001-10-16 Revised:2002-01-08 Published:2002-06-15
  • Contact: Shen He-Bai E-mail:shenhb@shtu.edu.cn

摘要: 利用原位电化学及表面增强拉曼散射(SERS)光谱方法对寡聚脱氧核苷酸(26-mers ODN和13-mers ODN)在银电极表面上的吸附状态进行了研究. 实验表明, 单链寡聚脱氧核苷酸在银电极上有很好的SERS光谱,单链寡聚脱氧核苷酸在银表面上主要以碱基腺嘌呤(A)为吸附点,吸附状态随电位变化而变化, 链长较短的寡聚脱氧核苷酸在银电极表面的吸附态对电位变化较敏感.

关键词: 寡聚脱氧核苷酸(ODN), 表面增强拉曼光谱, 腺嘌呤, 吸附, 银电极

Abstract: The oligodeoxynucleotides is anchoraged on the silver electrode surface by means of physical methods. The in situ Electrochemistry-Surface Enhanced Raman Scattering (SERS) Spectrum is used to study the adsorption state of 26-mers oligodeoxynucleotides (ODN) and 13-mers ODN on the electrode surface respectively. The SERS spectra of oligdeoxynucleotides based on the silver electrode have obtained. The results of study of the SERS spectra change of ODN with the change of potential on the silver electrode demonstrate that the oligodeoxynucleotides adsorbed on the silver electrode surface primarily through the special base-Adenine (A).The adsorption state changes with the variation of the potential on the electrode. The shorter ODN changes more quickly and is more sensitive to the change of potential than the longer ODN(see Table 1).

Key words: Oligodeoxynucleotide (ODN), SERS, Adenine, Adsorption, Silver electrodes