物理化学学报 >> 2002, Vol. 18 >> Issue (11): 1009-1013.doi: 10.3866/PKU.WHXB20021110

研究论文 上一篇    下一篇

四种抗癌物质对微管蛋白体外聚合及热变性的影响

周晓蕾;王保怀;李芝芬;曾慧慧   

  1. 北京大学物理化学研究所，北京　100871; 北京大学药学院，北京　100083
  • 收稿日期:2002-03-28 修回日期:2002-06-20 发布日期:2002-11-15
  • 通讯作者: 王保怀 E-mail:bhwang@pku.edu.cn

Studies of the Effects of Anti-tumor Compounds on the Assembly and Melting Denaturation of Tubulin in vitro

Zhou Xiao-Lei;Wang Bao-Huai;Li Zhi-Fen;Zeng Hui-Hui   

  1. Institute of Physical Chemistry, Peking University, Beijing　100871;School of Pharmaceutical Science, Peking University, Beijing,　100083
  • Received:2002-03-28 Revised:2002-06-20 Published:2002-11-15
  • Contact: Wang Bao-Huai E-mail:bhwang@pku.edu.cn

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摘要： 用浊度法和差示扫描量热法研究了四种小分子抗癌物质[紫杉醇、卡铂、猪芽藻提取物(ZYZ)和番荔枝素提取物(F43)]对微管蛋白体外聚合及热变性的影响。实验结果表明：当四种化合物的浓度均为15 μmol•L－1时，紫杉醇和猪芽藻提取物(ZYZ)能促进微管蛋白的聚合并使其热变性温度升高，而卡铂和番荔枝素提取物(F43)却对微管蛋白的聚合及热变性温度无明显影响。综合上述实验结果，对四种抗癌物质与微管蛋白相互作用的机理进行了讨论。

关键词: 微管, 紫杉醇, 卡铂, 猪芽藻提取物(ZYZ), 番荔枝素提取物(F43), 浊度, 差示扫描量热法(DSC)

Abstract: The effects of four kinds of anti-tumor compounds, taxol, carboplatin, ZYZ (the extractive from gleditsia), F43 (the extractive from squamocin), on the assembly and melting denaturation of tubulin in vitro were studied by turbidity and differential scanning calorimetry. The experimental results show that taxol and ZYZ enhance the assembly of tubulin, while carboplatin and F43 have no effect on the assembly of tubulin when the concentrations of all four kinds of compounds are 15 μmol•L－1.At the same time, taxol and ZYZ can make the melting points of tubuin rise, while the melting points of tubuin don't change in the presence of carboplatin and F43.The different interaction mechanisms of the compounds and tubulin have been discussed according to the above experimental results.

Key words: Tubulin, Taxol, Carboplatin, ZYZ, F43, Turbidity, DSC(differential scanning calorimetry)