物理化学学报 >> 2002, Vol. 18 >> Issue (12): 1125-1128.doi: 10.3866/PKU.WHXB20021214

研究简报 上一篇    

钙、镁离子对淀粉酶催化激活作用的热动力学

张洪林;于秀芳;张刚   

  1. 曲阜师范大学化学系，曲阜　273165；山东大学微生物技术国家重点实验室，济南　250100
  • 收稿日期:2002-04-08 修回日期:2002-07-03 发布日期:2002-12-15
  • 通讯作者: 张洪林 E-mail:Zhanghl@qfnu.edu.cn

Thermokinetics of Activation of Metal Ions to Amylase Catalyzed Reaction

Zhang Hong-Lin;Yu Xiu-Fang;Zhang Gang   

  1. Department of Chemistry, Qufu Normal University, Qufu　273165;　1State Key Laboratory of Microbial Technology, Shandong University, Jinan　250100
  • Received:2002-04-08 Revised:2002-07-03 Published:2002-12-15
  • Contact: Zhang Hong-Lin E-mail:Zhanghl@qfnu.edu.cn

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摘要： 利用热活性检测仪测定了无激活剂和有激活剂时淀粉在淀粉酶催化作用下的热功率－时间曲线，借助热动力学理论和对比进度法对曲线进行处理，得到了无激活剂时酶催化反应的米氏常数(Km)和最大速率(vmax)，以及有激活剂(Ca2＋,Mg2＋)时酶催化反应的表观米氏常数（Km1）和最大速率（vmax）.通过比较，得到了金属离子对酶催化反应定量的激活效果.

关键词: 微量热法, 热动力学, 对比进度, 淀粉酶, 催化反应

Abstract: Power－time curves of amylase catalyzed reaction with or without activation were obtained with a 2277 thermal activity monitor(Sweden). The data of Km and vmax were calculated for enzyme-catalyzed reactions without activation. Data of apparent Km1 and vmax were calculated for activation (Ca2＋, Mg2＋) using thermokinetics theory and reduced extent method. From the relationship between and concentration of metal ions(c), the effects of the activation were gained for metal ions of amylase-catalyzed reaction.

Key words: Microcalorimetry, Thermokinetics, Reduced extent, Amylase, Catalyzed reaction