物理化学学报 >> 2003, Vol. 19 >> Issue (08): 747-750.doi: 10.3866/PKU.WHXB20030815

研究论文 上一篇    下一篇

二价金属离子对磷脂聚集体的影响

俞科兵;杨展澜;张莉;王凡;翁诗甫;吴瑾光   

  1. 北京大学化学与分子工程学院,化学生物学系,稀土材料化学及应用国家重点实验室,北京 100871
  • 收稿日期:2003-01-23 修回日期:2003-04-07 发布日期:2003-08-15
  • 通讯作者: 杨展澜 E-mail:yzl@chem.pku.edu.cn

Influence of Divalent Metal Ions on the Aggregation Properties of EYPC

Yu Ke-Bing;Yang Zhan-Lan;Zhang Li;Wang Fan;Weng Shi-Fu;Wu Jin-Guang   

  1. State Key Laboratory of Rare Earth Materials Chemistry and Applications, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871
  • Received:2003-01-23 Revised:2003-04-07 Published:2003-08-15
  • Contact: Yang Zhan-Lan E-mail:yzl@chem.pku.edu.cn

摘要: 通过浊度分析和激光光散射光谱研究了二价金属离子(Ca2+、Cu2+和Mn2+)对蛋黄卵磷脂(EYPC)聚集态的影响.结果表明,自由的二价金属离子对EYPC囊泡有破坏作用,并使EYPC囊泡发生相转变,形成胶束;而与牛磺胆酸钠(TC)结合的二价金属离子使EYPC囊泡半径减小,但不破坏EYPC囊泡.

关键词: 卵磷脂(PC), 二价金属离子, 牛磺胆酸钠(TC), 聚集态

Abstract: It is well known that phosphatidylcholine (PC) vesicles are predominant carriers of cholesterol in human bile. Although the interaction of bile salts with PC has been extensively studied, relatively little attention has been given to effects of divalent metal ions on the aggregation properties of PC. We used both light scattering and UV spectroscopies to follow the effects of divalent metal ions on EYPC (egg yellow PC) vesicles. We conclude that when divalent metal ions existed, the mixed vesicles formed by PC and TC (sodium taurocholate) with the ratio of 1:3 were more stable. Free divalent metal ions act to precipitate unilamellar PC vesicles whereas, in combination with sodium taurocholate, these ions act to decrease the size of PC vesicles and induce a phase transition from PC vesicles to mixed micelles. It is a possible reason for gallstones formation when extra free divalent metal ions exist in human bile.

Key words: Phosphatidylcholine, Divalent metal ions, Sodium taurocholate,  Aggregation properties