物理化学学报 >> 2003, Vol. 19 >> Issue (09): 879-882.doi: 10.3866/PKU.WHXB20030921

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DNA在纳米金标上的组装、杂交、检测与银增强

王美佳;纪小会;王连英;刘敏;刘艳梅;白玉白;李铁津;李景虹   

  1. 吉林大学化学学院, 长春 130023; 1中国科学院长春应用化学研究所, 长春 130022
  • 收稿日期:2003-03-17 修回日期:2003-04-21 发布日期:2003-09-15
  • 通讯作者: 白玉白 E-mail:yubai@jlu.edu.cn

The Assembly, Hybridization and Detection of DNA on Gold Nanoparticles with Silver Enhancement

Wang Mei-Jia;Ji Xiao-Hui;Wang Lian-Ying;Liu Min;Liu Yan-Mei;Bai Yu-Bai;Li Tie-Jin;Li Jing-Hong   

  1. College of Chemistry, Jilin University, Changchun 130023;1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022
  • Received:2003-03-17 Revised:2003-04-21 Published:2003-09-15

摘要: 利用电化学方法进行DNA的杂交检测.将目标ss-DNA固定在玻碳电极表面, 使其与纳米金标记的互补DNA发生杂化反应, 通过银增强试剂(该种试剂可以使银在纳米金表面沉积, 达到信号增强的效果)在纳米金上沉积银, 形成银包金的核壳结构.在酸性介质中沉积的银被氧化释放, 以离子状态存在于溶液中.用阳极溶出伏安法(ASV)检测银离子从而达到间接检测目标DNA的目的.测定结果表明,ss-DNA的浓度在100~1 000 pmol•L-1 范围内有非常好的线性关系, 检测限为10 pmol•L-1.

关键词: DNA, 纳米金标记的DNA, 杂化, 银增强, 阳极溶出伏安法

Abstract: Authors report on the detection of DNA hybridization using electrochemistry method. The target ss-DNA was immobilized on glassy carbon electrode and hybridized with the gold nanoparticle-oligonucleotide DNA probe. A nanoparticle-promoted Ag precipitation was used to enlarge the nanoparticle tag, forming the Au/Ag core-shell structure, followed by the release of the silver metal atoms anchored on the hybrids by oxidative metal dissolution and the indirect determination of the solubilized Ag ions by anodic stripping voltammetry (ASV) at a carbon fiber microelectrode. Further signal amplification, and lowering of the detection limits to the picomolar domains, was achieved by precipitating silver. The results show that this method has good correlation for detection of DNA in the range of 100~1 000 pmol•L-1and allows detection at levels as low as 10 pmol•L-1 of the target oligonucleotides.

Key words: DNA, Gold nanoparticle-oligonucleotide DNA probe, Hybridization, A nanoparticle-promoted Ag precipitation, Anodic stripping voltammetry (ASV)