物理化学学报 >> 2004, Vol. 20 >> Issue (10): 1182-1185.doi: 10.3866/PKU.WHXB20041003

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用于识别不同细胞蛋白质组的噬菌体抗体芯片

洪龙;廖玮;魏芳;赵新生;朱圣庚   

  1. 北京大学生命科学学院; 分子动态与稳态结构国家重点实验室, 北京大学化学与分子工程学院化学生物学系,北京 100871
  • 收稿日期:2004-07-07 修回日期:2004-07-30 发布日期:2004-10-15
  • 通讯作者: 赵新生 E-mail:zhaoxs@pku.edu.cn

Phage Antibody Chip for Discriminating Proteomes from Different Cells

Hong Long;Liao Wei;Wei Fang;Zhao Xin-Sheng;Zhu Sheng-Geng   

  1. School of Life Science, State Key Laboratory of Molecular Dynamic and Stable Structures, and Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871
  • Received:2004-07-07 Revised:2004-07-30 Published:2004-10-15
  • Contact: Zhao Xin-Sheng E-mail:zhaoxs@pku.edu.cn

摘要: 将4个鼠源噬菌体抗体克隆和1个人源噬菌体抗体克隆偶联到羧基终止的硅片表面,制成分析型模型芯片.挑选健康人体淋巴细胞为正常细胞的代表, HeLa细胞为肿瘤细胞的代表,提取细胞的全部蛋白质并用荧光染料Cy3标记,与制成的分析芯片反应,得到了不同的结合图谱.实验结果表明,以噬菌体抗体为分子感受器的分析芯片可用于识别不同细胞的蛋白质组.

关键词: 噬菌体抗体库, 生物传感器, 噬菌体抗体芯片, 蛋白质组

Abstract: A model analytical chip was prepared by coupling 4 phage antibody clones from mouse and 1 clone from human to the surface of carboxyl-terminated silicon. The lymphocytes from healthy donors were selected as normal cells, while HeLa cells were selected as tumor cell. The whole proteins were labeled by fluorescent dye Cy3 after isolation from lymphocytes and HeLa cells, and then reacted with the analytical chip. A different binding pattern was obtained. Our results showed that the protein chip made of phage antibodies was able to recognize the proteome from different cells.

Key words: Phage antibody library, Biosensors, Phage antibody chip, Proteome