物理化学学报 >> 2005, Vol. 21 >> Issue (08): 862-866.doi: 10.3866/PKU.WHXB20050808

研究论文 上一篇    下一篇

Tween-20与牛血清白蛋白的相互作用

刘静; 徐桂英; 刘军; 于丽; 张洪林; 于秀芳   

  1. 山东大学胶体与界面化学教育部重点实验室,济南 250100; 曲阜师范大学化学系,山东 曲阜 273165; 河北医科大学药学院,石家庄 050017
  • 收稿日期:2004-12-24 修回日期:2005-02-16 发布日期:2005-08-15
  • 通讯作者: 徐桂英 E-mail:xuguiying@sdu.edu.cn

Interaction between Tween-20 and BSA

LIU Jing; XU Gui-ying; LIU Jun; YU Li; ZHANG Hong-lin; YU Xiu-fang   

  1. Key Laboratory of Colloid and Interface Chemistry Ministry of Education, Shandong University, Jinan 250100; Department of Chemistry, Qufu Normanl University, Qufu 273165; Pharmic Institute, Hebei Medical University, Shijiazhuang 050017
  • Received:2004-12-24 Revised:2005-02-16 Published:2005-08-15
  • Contact: XU Gui-ying E-mail:xuguiying@sdu.edu.cn

摘要: 通过表面张力和等温滴定量热方法,研究了非离子表面活性剂Tween-20与牛血清白蛋白(BSA)的相互作用. 结果表明,BSA与Tween-20的相互作用较弱,当Tween-20浓度增大时,能将吸附于表面上的BSA分子取代. BSA/Tween-20混合体系的性质随温度的变化趋势与单一非离子型表面活性剂相似. BSA浓度在3.7×10-5~11.1×10-5 kg•L-1范围内,Tween-20的cmc不随BSA浓度的变化而变化. 当Tween-20的含量一定时,Tween-20与BSA相互作用过程的热效应也与BSA 的浓度无关. BSA/Tween-20混合体系的热力学参数表明, BSA的存在使Tween-20的cmc减小,体系的熵变增大.

关键词: Tween-20, BSA, 表面张力, 等温量热滴定, 相互作用

Abstract: The interaction between Tween-20 and BSA was studied by surface tension and isothermal titration microcalorimetry. The results show that there is a weak interaction between Tween-20 and BSA and the Tween-20 molecules can replace BSA molecules adsorbed on the surface. A competition adsorption between Tween-20 and BSA occurred at the surface of mixed solution. The tendency of change of the properties of the BSA/Tween-20 mixed system with temperature is similar to that of the single non-ionic surfactant. In the range of BSA concentration from 3.7×10-5 kg•L-1 to 1.1×10-5 kg•L-1, the cmc of Tween-20 does not change with BSA concentrations. When the amount of Tween-20 is fixed, the thermal effects of the interaction between BSA and Tween-20 does not depend on the BSA concentration. The thermodynamic parameters of the mixed solutions in the presence or absence of BSA indicate that the cmc of Tween-20 decreases and the value of entropy change of the system increases in the presence of BSA in solution.

Key words: Tween-20, BSA, Surface tension, Isotherm titration microcalorimetry, Interaction