物理化学学报 >> 2005, Vol. 21 >> Issue (10): 1091-1095.doi: 10.3866/PKU.WHXB20051006

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钯(Ⅱ)三元配合物稳定性及其与DNA作用研究

高恩君; 丁丽娜; 刘祁涛; 孙亚光   

  1. 沈阳化工学院化工系, 沈阳 110142； 辽宁大学化学科学与工程学院, 沈阳 110036
  • 收稿日期:2005-02-02 修回日期:2005-04-11 发布日期:2005-10-15
  • 通讯作者: 高恩君 E-mail:ejgao@yahoo.com.cn

Studies on Stability of Pd(Ⅱ)Ternary Complexes and Interaction with DNA

GAO En-jun; DING Li-na; LIU Qi-tao; SUN Ya-guang   

  1. Chemistry Engineering Department, Shenyang Institute of Chemical Technology, Shenyang 110142； 1Chemical Institute of Science and Technology, Liaoning University, Shenyang 110036
  • Received:2005-02-02 Revised:2005-04-11 Published:2005-10-15
  • Contact: GAO En-jun E-mail:ejgao@yahoo.com.cn

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摘要： 合成了[Pd(bipy)(DL-gly)]Cl•2H22O(2)两个钯三元配合物. 配合物１和２的稳定常数对数值lgβ分别为13.81和13.71, 表征常数ΔlgK、lgX高于统计值, 表明在配合物分子内存在d-p π电子协同效应. 紫外光谱、荧光光谱结果表明, 配合物１和２与鱼精DNA主要以插入方式键合, 键合常数分别为2.37×106(1)和4.57×106(2). CD光谱图也显示, 配合物与DNA分子之间发生了作用. 质粒pBR322 DNA的凝胶电泳图表明, 配合物浓度在3.0０×10-3～7.5０×10-4 mol •L-1范围内对DNA分子有切割作用, 配合物浓度低于3.75×10-4 mol •L-1时则主要以插入方式与DNA键合.

关键词: 钯(Ⅱ)三元配合物, 稳定性, 联吡啶, 氨基酸, DNA, 插入作用

Abstract: Two ternary complexes [Pd(bipy)(DL-gly)]Cl•2H2O(1) and [Pd(bipy)(L-lys)]Cl•H2O(2) were synthesized. The stability constants lgβ of two complexes are 13.81(1) and 13.71(2), respectively, and relative stability ΔlgK、lgX are higher than statistical value. The results show that there exists d-p π electronic effect in complexes．Intercalation of the complexes with DNA can be demonstrated by UV-Vis spectra and fluorescence spectra．The binding constsns of the two complexes with sperm DNA are 2.37×106(1) and 4.57×106(2), respectively. It shows that the interaction intensity is 1＜2. The CD spectra also show that the tow complexes were reacted with DNA．The results of gel electrophoresis experiments reveal that plasmid pBR322 DNA was cleaved by the two complexes when complex concentration ranges from 3.00×10-3 to 7.50×10-4 mol •L-1, whereas insertion is main binding model when complex concentration is lower than 3.75×10-4 mol •L-1.

Key words: Palladium(Ⅱ) ternary complexes, Stability, Bipyridine, Amino acid, DNA, Insertion action