物理化学学报 >> 2006, Vol. 22 >> Issue (10): 1248-1252.doi: 10.3866/PKU.WHXB20061015

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钛/活细胞界面原位EIS研究

胡仁;林理文;张帆;林昌健   

  1. 厦门大学化学化工学院化学系, 固体表面物理化学国家重点实验室, 福建 厦门 361005
  • 收稿日期:2006-03-13 修回日期:2006-05-29 发布日期:2006-10-11
  • 通讯作者: 林昌健 E-mail:cjlin@xmu.edu.cn

In situ EIS Study on Titanium/Living Cells Interface

HU Ren;LIN Li-Wen;ZHANG Fan;LIN Chang-Jian   

  1. State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Eugineering, Xiamen University, Xiamen 361005, P. R. China
  • Received:2006-03-13 Revised:2006-05-29 Published:2006-10-11
  • Contact: LIN Chang-Jian E-mail:cjlin@xmu.edu.cn

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摘要： 设计了原位电解池用于生物材料/活细胞界面电化学交流阻抗谱(EIS)的测量, 原位观测了钛/MG63细胞界面EIS行为及其随时间的变化. 原位电解池中引入Ag/AgCl作为参比电极, 解决了EIS测量过程中高频部分信号失真的问题. 钛/MG63细胞界面EIS研究初步结果表明: 活细胞在钛电极表面附着, 可改变界面双电层结构和电极的表面状态, 进而影响界面的电化学性质. 实验发现, 由于钛电极表面氧化层的阻抗信号处于中低频段, 而细胞膜层自身的电化学阻抗响应位于高频范围, 由此, 可分别分析不同的电极过程, 解析其相互关系, 研究活细胞在生物材料表面的作用机理.

关键词: 钛/细胞界面, 原位EIS, Ag/AgCl参比电极

Abstract: Using a home-made in situ electrochemical cell of electrochemical impedance spectroscop(EIS), the impedance behaviors of the titanium/MG63 cells interface were measured to elucidate the interfacial structure and to monitor the interactions of biomaterials and living cells in a physiological condition. The in situ electrochemical cell was designed to detect the dynamic EIS behavior of living cells growing on a tiatanium surface. A three-well design of electrochemical cell was used to decrease system error and a home-made Ag/AgCl electrode was introduced as a reference electrode to eliminate the distortion phenomena in high frequency range. The EIS spectra and its time dependency of titanium/MG63 cells interface showed that the polarization resistance was increased with the decreasing of CPE constant (Y0) and a new time constant in high frequency range was detected. It suggested that the adhesion and growth activities of the living cells on the biomaterial surface may influence the surface status and the structure of the double layer at the interface, and thus alter its electrochemical characteristics.

Key words: Titanium/cells interface, in situ EIS, Ag/AgCl reference electrode