物理化学学报 >> 2007, Vol. 23 >> Issue (09): 1483-1486.doi: 10.3866/PKU.WHXB20070934

研究简报 上一篇    

AOT/Triton X-100混合反胶束体系中假丝酵母脂肪酶催化蓖麻油水解的活性

张玉霞; 赵继华; 杜中玉; 方建; 安学勤; 沈伟国   

  1. 兰州大学化学化工学院, 兰州 730000; 南京师范大学化学与环境科学学院, 南京 210097; 华东理工大学化学系, 上海 200237
  • 收稿日期:2007-03-22 修回日期:2007-04-25 发布日期:2007-09-06
  • 通讯作者: 安学勤 E-mail:anxueqin@163.com

Enzymatic Activity of Candida Rugosa Lipase in an AOT/Triton X-100 Mixed Reverse Micellar System

ZHANG Yu-Xia; ZHAO Ji-Hua; DU Zhong-Yu; FANG Jian; AN Xue-Qin; SHEN Wei-Guo   

  1. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China; College of Chemistry and Environment Science, Nanjing Normal University, Nanjing 210097, P. R. China; Department of Chemistry, East China University of Science and Technology, Shanghai 200237, P. R. China
  • Received:2007-03-22 Revised:2007-04-25 Published:2007-09-06
  • Contact: AN Xue-Qin E-mail:anxueqin@163.com

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摘要： 研究了二(2-乙基己基)琥珀酸磺酸钠(AOT)/Triton X-100混合反胶束体系中假丝酵母脂肪酶(candida rugosa lipase)催化蓖麻油水解的反应. 考察了Triton X-100占总表面活性剂的摩尔分数(x(Triton X-100))、水与总体表面活性剂的摩尔比(ω0)、pH值、反应温度以及底物蓖麻油的浓度等因素对酶活性的影响. 研究结果表明, 加入非离子表面活性剂Triton X-100可以使假丝酵母脂肪酶的活性得到显著提高, 但是当底物蓖麻油的浓度大于0.24 mol·L-1时, 会对假丝酵母脂肪酶产生抑制作用.

关键词: 混合反胶束, 假丝酵母脂肪酶, 蓖麻油, AOT, Triton X-100

Abstract: The catalytic activity of candida rugosa lipase has been investigated in sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/Triton X-100 mixed reverse micellar systemby using castor oil as substrate. The effects of molar fraction of Triton X-100 in total surfactants, molar ratio of water to total surfactants, reaction temperature, pH and the concentration of castor oil on enzyme activity were studied when the concentration of overall surfactant was kept constant. Significant increase of the activity of candida rugosa lipase was observed in the presence of Triton X-100. It was also found that the castor oil inhibited the reaction when its concentration was higher than 0.24 mol·L-1.

Key words: Mixed reverse micelles, Candida rugosa lipase, Castor oil, AOT, Triton X-100

MSC2000: 

  • O648