物理化学学报 >> 2009, Vol. 25 >> Issue (07): 1285-1289.doi: 10.3866/PKU.WHXB20090708

研究论文 上一篇    下一篇

块状壳聚糖多孔支架内交替浸渍沉积磷灰石层

黄志海, 董寅生, 林萍华   

  1. 东南大学江苏省先进金属材料高技术研究重点实验室, 南京 211189|河海大学材料科学与工程学院, 南京 210098
  • 收稿日期:2009-01-30 修回日期:2009-03-18 发布日期:2009-06-26
  • 通讯作者: 董寅生 E-mail:dongys@seu.edu.cn

Depositing an Apatite Coating in/on Massive Chitosan Porous Scaffolds by an Alternate Soaking Method

HUANG Zhi-Hai, DONG Yin-Sheng, LIN Ping-Hua   

  1. Jiangsu Key Laboratory of Advanced Metallic Materials, Southeast University, Nanjing 211189, P. R. China|School of Materials Science and Engineering, Hehai University, Nanjing 210098, P. R. China
  • Received:2009-01-30 Revised:2009-03-18 Published:2009-06-26
  • Contact: DONG Yin-Sheng E-mail:dongys@seu.edu.cn

摘要:

在聚合物支架内沉积羟基磷灰石涂层有望提高支架的生物活性和骨传导性. 本研究采用交替浸渍沉积法, 以块状壳聚糖(Cs)三维多孔支架为沉积模板, 在氯化钙溶液和磷酸氢二钠溶液中交替浸渍, 沉积了羟基磷灰石(HA)涂层. 应用XRD、FT-IR、SEM、孔隙率测试、焙烧法和压缩实验对沉积前后支架的组成、形貌、孔隙率、无机物沉积量以及压缩强度进行了表征. 研究结果表明, 支架上沉积物为低结晶度的碳酸羟基磷灰石, 沿c轴择优生长, 与天然骨中磷灰石类似. 扫描电镜照片显示, 磷灰石在支架孔壁上的沉积量呈梯度分布, 外部沉积量多于内部, 靠近支架表面孔隙部分堵塞, 但内部仍保持连通的孔隙结构. 经6次交替浸渍处理的支架, 孔隙率为94.0%, 羟基磷灰石沉积量达到总质量的13.5%, 压缩强度则由0.055 MPa提高到0.109 MPa.

关键词: 羟基磷灰石, 壳聚糖, 多孔支架, 交替浸渍, 复合材料

Abstract:

We hope to improve the biological activity and the osteo-conductivity of a porous polymer scaffold by the deposition of a hydroxyapatite (HA) coating.We used an alternate soaking deposition method to prepare HAcoating. A massive chitosan (Cs) porous scaffold was used as a deposition template and immersed alternately in calciumchloride and disodium hydrogen phosphate solutions. Finally, a hydroxyapatite coating was deposited in/on the massive chitosan porous scaffold. The compositions, micromorphology, porosity, inorganic deposition amount and compressive strength were characterized by XRD, FT-IR, SEM, porosity measurements, calcination and a compression experiment. Results indicated that the deposition mineral was a low crystallinity carbonate hydroxyapatite with c axis preferential growth and was similar to hydroxyapatite in natural bone. SEMresults showed that the sedimentary hydroxyapatite had a gradient distribution in the scaffold. More deposition was observed on the outer pore wall surface than the inner pore wall surface. The exterior pores of the scaffold were partially blocked but the inner pore structure of the scaffold was still interconnected. The porosity of the deposited scaffold was 94.0% and the deposited hydroxyapatite was 13.5% of the total mass after 6 alternate soaking cycles. The compressive strength of the scaffold increased from 0.055 MPa to 0.109 MPa.

Key words: Hydroxyapatite, Chitosan, Porous scaffold, Alternate soaking, Composite material

MSC2000: 

  • O647