物理化学学报 >> 2010, Vol. 26 >> Issue (02): 304-310.doi: 10.3866/PKU.WHXB20100141

结构化学 上一篇    下一篇

β-乳球蛋白在磁化水中的水合作用: 磁化处理对水分子缔合构造及蛋白质水合特性的影响

和劲松, 杨宏伟, 蔡然, 罗之纲, 祝万鹏   

  1. 清华大学环境科学与工程系, 北京 100084; 康师傅控股有限公司中央研究所, 天津 300457
  • 收稿日期:2009-08-09 修回日期:2009-10-28 发布日期:2010-01-26
  • 通讯作者: 和劲松, 杨宏伟 E-mail:hejinsong@mail.tsinghua.edu.cn; yanghw@tsinghua.edu.cn

Hydration of β-Lactoglobulin in Magnetized Water: Effect of Magnetic Treatment on the Cluster Structure of Water and Hydration Properties of Proteins

HE Jin-Song, YANG Hong-Wei, CAI Ran, LO Chih-Kang, ZHU Wan-Peng   

  1. Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, P. R. China; Central Research Institute, Ting Hsin International Group, Tianjin 300457, P. R. China
  • Received:2009-08-09 Revised:2009-10-28 Published:2010-01-26
  • Contact: HE Jin-Song, YANG Hong-Wei E-mail:hejinsong@mail.tsinghua.edu.cn; yanghw@tsinghua.edu.cn

摘要:

为探讨蛋白质在磁化水中的水合作用, 首先利用粘度测定及氧17核磁共振(17O-NMR)对经静磁场(MF)处理不同有效时间(teff)后的纯水进行了分析, 进一步又利用差示扫描热量计(DSC)及NMR对溶解于磁化水的β-乳球蛋白(β-Lg)的水合特性进行了分析. 随teff的增加, 水分子的内能不断减小, 处于氢键结合状态的水分子的比例不断增加. 结果表明MF处理促进了水分子缔合结构的形成, 这一点可能与氢键的形成有关. 随teff的增加, β-Lg表面水分子的运动性没有明显变化, 但β-Lg溶液中非自由结合水的含量不断增加. 说明β-Lg的水合作用与水分子的缔合分布有关, 该分布依存于水分子的氢键状态并可通过磁场处理加以改变.

关键词: 磁化, 氢键, 水分子缔合, 蛋白质水合

Abstract:

To investigate the hydration of proteins in magnetized water, pure water was treated with a static magnetic field (MF) over different effective treatment time (teff). Viscosimetry and oxygen nuclear magnetic resonance (17O-NMR) spectra were recorded. The hydration properties of β-lactoglobulin (β-Lg) in magnetized water were examined by differential scanning calorimetry (DSC) and NMR. By increasing teff, the internal energy of the water decreased and the concentration of“hydrogen-bonded”water increased. This result indicates that MF treatment promotes the formation of water clusters, which can be attributed to an increase in hydrogen bonding. For the β-Lg solution prepared in magnetized water, the mobility of water molecules around the β-Lg surface did not change significantly with an increase in teff but the quantity of non-freezable bound water increased. This suggests that β-Lg hydration is related to the formation of water clusters, which depends on the hydrogen-bonding structure of water and can be varied by MF treatment.

Key words: Magnetization, Hydrogen bond, Water molecule cluster, Protein hydration

MSC2000: 

  • O641