物理化学学报 >> 2010, Vol. 26 >> Issue (09): 2468-2472.doi: 10.3866/PKU.WHXB20100923

光化学与光谱 上一篇    下一篇

金纳米粒子比色测定细胞色素c构象变化

栗娜, 周剑章, 林玲玲, 韩楠楠, 林仲华   

  1. 厦门大学化学化工学院化学系,固体表面物理化学国家重点实验室, 福建厦门361005
  • 收稿日期:2010-03-08 修回日期:2010-04-22 发布日期:2010-09-02
  • 通讯作者: 周剑章 E-mail:jzzhou@xmu.edu.cn
  • 基金资助:

    国家自然科学基金(20603027, 20423002)资助项目

Au Nanoparticles Based Colorimetric Detection of Conformational Changes in Cytochrome c

LI Na, ZHOU Jian-Zhang, LIN Ling-Ling, HAN Nan-Nan, LIN Zhong-Hua   

  1. Key Laboratory of Physical Chemistry of Solid Surface, Department of Chemistry, College of Chemistry and Chemistry Engineering, Xiamen University, Xiamen 361005, Fujian Province, P. R. China
  • Received:2010-03-08 Revised:2010-04-22 Published:2010-09-02
  • Contact: ZHOU Jian-Zhang E-mail:jzzhou@xmu.edu.cn
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (20603027, 20423002).

摘要:

金纳米粒子(Au NPs)的颜色会随着细胞色素c (Cyt c)构象变化而发生较大的变化, 作者在改变原测定流程的基础上利用这种有规律的变化研究和测定了H+和L-半胱氨酸(L-Cys)对Cyt c 的构象变化. 实验中分别加入pH=1-13 的Cyt c, 可以使Au NPs显示青、蓝、紫、红等明显不同的颜色, 从而可以利用Au NPs 比色快速测定不同pH 值的Cyt c 构象变化. 在pH=7 时, 当附加L-Cys 浓度从低浓度变化到高浓度时, Au NPs 颜色逐渐从紫色变化到蓝色、青色, 从而实现利用Au NPs 比色测定由L-Cys 所引起Cyt c 构象变化. 圆二色(CD)光谱证实了pH=1-13 和不同浓度L-Cys 下的Cyt c 构象变化. 借助紫外-可见吸收光谱和扫描电子显微镜(SEM)进一步明确了加入Cyt c后Au NPs的不同聚集状态与其颜色变化的关系.

关键词: Au 纳米粒子, 比色测定, Cyt c 构象变化, pH值, L-Cys

Abstract:

The colors of Au nanoparticles (Au NPs) change along with conformational changes in cytochrome c (Cyt c). We exploited this property for the colorimetric detection of Cyt c conformational changes induced by H+ and L-cysteine (L-Cys). We improved the conventional procedure for this detection. After the addition of Cyt c within different pH values, the Au NPs are either cyan, blue, purple or red. This indicates that the Au NPs can be applied to the rapid colorimetric detection of pH-induced conformational changes in Cyt c. At pH 7 the color of Au NPs changes from purple to blue and then cyan upon the addition of L-Cys, which suggests that the Au NPs can be used for the colorimetric detection of Cyt c conformational changes caused by interaction with L-Cys. The conformational changes of Cyt c were verified by circular dichroism (CD) spectroscopy. The relationship between the aggregation states and colors of the Au NPs after the addition of Cyt c was characterized by UV-Vis absorption spectroscopy and scanning electron microscopy (SEM).

Key words: Au nanoparticle, Colorimetric detection, Conformational changes of Cyt c, pH value, L-Cys

MSC2000: 

  • O648