物理化学学报 >> 2010, Vol. 26 >> Issue (11): 2997-3001.doi: 10.3866/PKU.WHXB20101008

胶体及界面化学 上一篇    下一篇

N-烷基-N,N-二(2-羟乙基)-N-甲基溴化铵与牛血清白蛋白的相互作用

邵爽1, 胡晓环2, 游金宗1, 雷群芳2   

  1. 1. 浙江外国语学院化学系, 杭州310012;
    2. 浙江大学化学系, 杭州310027
  • 收稿日期:2010-04-13 修回日期:2010-07-05 发布日期:2010-10-29
  • 通讯作者: 邵爽, 雷群芳 E-mail:sshao@zjei.net, qflei@zju.edu.cn
  • 基金资助:

    国家自然科学基金(20673098)和浙江省自然科学基金(Y4080201)资助项目

Interactions between N-Alkyl-N,N-di(2-hydroxyethyl)-N-methylammonium Bromide and Bovine Serum Albumin

SHAO Shuang1, HU Xiao-Huan2, YOU Jin-Zong1, LEI Qun-Fang2   

  1. 1. Department of Chemistry, Zhejiang International Studies University, Hangzhou 310012, P. R. China;
    2. Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China
  • Received:2010-04-13 Revised:2010-07-05 Published:2010-10-29
  • Contact: SHAO Shuang, LEI Qun-Fang E-mail:sshao@zjei.net, qflei@zju.edu.cn
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (20673098) and Natural Science Foundation of Zhejiang Province, China (Y4080201).

摘要:

用荧光光谱法在298 K 研究了Tris-HCl缓冲溶液(pH=7.1)中系列N-烷基-N,N-二(2-羟乙基)-N-甲基溴化铵(烷基链长为C12到C16)与牛血清白蛋白(BSA)的结合作用, 考察了表面活性剂结构、BSA 浓度对结合作用的影响,分别用Stern-Volmer 方程、虚拟结合常数模型探讨了表面活性剂在浓度较低区域与BSA 的作用机制. 结果表明: 三种季铵盐表面活性剂均对BSA 内源荧光有猝灭作用, 并导致其最大发射波长蓝移; 表面活性剂的烷基链越长, Stern-Volmer 猝灭常数和虚拟结合常数越大,表面活性剂与BSA的结合作用也越强.

 

关键词: 季铵盐表面活性剂, 牛血清白蛋白, 荧光猝灭, 疏水作用, 虚拟结合常数

Abstract:

Interactions between a series of N-alkyl-N,N-di(2-hydroxyethyl)-N-methylammonium bromides with alkyl chain from dodecyl to cetyl and bovine serum albumin (BSA) in Tris-HCl buffer solution (pH=7.1) were studied by fluorescence spectroscopy at 298 K. Effects of the surfactant structure and BSA concentration on the interactions were examined. The interaction mechanism between BSA and surfactants at a relatively low concentration range was discussed using the Stern-Volmer equation and a model of the pseudo association constant. Each of the quaternary ammonium surfactants has a fluorescence quenching effect on BSA and leads to a blue shift of the maximum emission wavelength. The Stern-Volmer quenching constant and the pseudo association constant increase, which indicates that the interactions between surfactants and BSA become stronger, as the hydrophobic chain lengths increase.

 

Key words: Quaternary ammoniumsurfactant, Bovine serumalbumin, Fluorescence quenching, Hydrophobic interaction, Pseudo association constant

MSC2000: 

  • O647