物理化学学报 >> 2011, Vol. 27 >> Issue (03): 722-728.doi: 10.3866/PKU.WHXB20110235

材料物理化学 上一篇    下一篇

水溶性银纳米颗粒的制备及抗菌性能

孙磊1, 刘爱心1, 黄红莹2, 陶小军1, 赵彦保1, 张治军1   

  1. 1. 河南大学特种功能材料教育部重点实验室, 河南 开封 475004;
    2. 河南大学医学院, 河南 开封 475004
  • 收稿日期:2010-08-24 修回日期:2010-11-01 发布日期:2011-03-03
  • 通讯作者: 孙磊 E-mail:sunlei@henu.edu.cn
  • 基金资助:

    国家自然科学基金(50701016)和河南省教育厅自然科学基金(2007150008, 2008B150003)资助项目

Preparation and Antibacterial Properties of Water-Soluble Ag Nanoparticles

SUN Lei1, LIU Ai-Xin1, HUANG Hong-Ying2, TAO Xiao-Jun1, ZHAO Yan-Bao1, ZHANG Zhi-Jun1   

  1. 1. Key Laboratory for Special Functional Materials of Ministry of Education, Henan University, Kaifeng 475004, Henan Province, P. R. China;
    2. Medical College of Henan University, Kaifeng 475004, Henan Province, P. R. China
  • Received:2010-08-24 Revised:2010-11-01 Published:2011-03-03
  • Contact: SUN Lei E-mail:sunlei@henu.edu.cn
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (50701016) and Natural Science Foundation of Education Department of Henan Province, China (2007150008, 2008B150003).

摘要:

采用液相还原法, 以单宁酸为还原剂, 聚乙烯吡咯烷酮(PVP)为修饰剂制备出了水溶性的表面修饰Ag纳米颗粒. 通过X 射线粉末衍射仪(XRD)、透射电子显微镜(TEM)、紫外-可见吸收分光光度计(UV-Vis)、傅里叶变换红外(FTIR)光谱仪等对所得样品的形貌和结构进行了表征. 采用肉汤稀释法测试了样品的抗菌性能, 考察了样品在水相中的分散稳定性, 提出了PVP修饰Ag纳米颗粒的形成机理. 结果表明所制备的样品具有Ag的面心立方晶体结构, 平均粒径为15-17 nm. 样品在水相中能长时间稳定分散; 对埃希氏大肠杆菌(E. coli)、金黄色葡萄球菌(S. aureus)具有明显的抗菌作用. 操作简便、条件温和的制备方法易于在工业规模上放大; 试剂无毒, 使得所制备的PVP修饰Ag纳米颗粒作为抗菌剂具有良好的应用前景.

关键词: 银纳米颗粒, 水溶性, 表面修饰, 绿色无毒, 抗菌性

Abstract:

Water-soluble surface modified silver nanoparticles were synthesized by liquid phase reduction with tannic acid as the reductant and polyvinyl pyrrolidone (PVP) as the surface modification agent. The structure and morphology of the as-synthesized powders were investigated by X-ray powder diffraction (XRD), transmission electron microscopy (TEM), ultraviolet-visible (UV-Vis) absorption spectroscopy, and Fourier-transform infrared (FTIR) spectrometry. The antibacterial activity of the water- soluble Ag nanoparticles against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was investigated by broth dilution. The stable dispersion duration of the as-synthesized Ag nanoparticles in water was also determined. A mechanism for PVP modified Ag nanoparticle formation is proposed. The results show that the as-synthesized PVP modified Ag nanoparticles have a face-centered cubic crystalline structure. The average diameter of the Ag nanoparticles ranges from 15 to 17 nm. The as- synthesized powders have good solubility in water over a long period of time. PVP modified Ag nanoparticles exhibit good antibacterial properties against E. coli and S. aureus. This simple and mild preparation method can be easily increased to an industrial scale process and, therefore, PVP modified Ag nanoparticles are potentially a new type of antibacterial.

Key words: Ag nanoparticles, Water-soluble, Surface modification, “Green&rdquo, and nontoxic, Antibacterial property

MSC2000: 

  • O648