物理化学学报 >> 2011, Vol. 27 >> Issue (10): 2469-2477.doi: 10.3866/PKU.WHXB20110939

材料物理化学 上一篇    下一篇

多壁碳纳米管负载Fe3O4磁性纳米粒子表面吸附增强过氧化酶的催化活性

高云燕1, 李海霞1, 欧植泽1, 郝平1, 李嫕2, 杨国强3   

  1. 1. 西北工业大学理学院, 空间应用物理与化学教育部重点实验室, 西安 710072;
    2. 中国科学院理化技术研究所, 光化学转换与功能材料重点实验室, 北京 100190;
    3. 中国科学院化学研究所, 光化学重点实验室, 北京 100190
  • 收稿日期:2011-04-10 修回日期:2011-06-29 发布日期:2011-09-27
  • 通讯作者: 高云燕, 欧植泽 E-mail:gaoyunyan@nwpu.edu.cn; ouzhize@nwpu.edu.cn
  • 基金资助:

    国家自然科学基金(21073143), 教育部春晖计划基金(Z2009-1-71002, Z2009-1-71006)及西北工业大学基础研究基金(JC200822, JC20100239)资助项目

Enhancing the Catalytic Activity of Peroxidase by Adsorption onto Fe3O4 Magnetic Nanoparticle/Multiwalled Carbon Nanotube Composite Surfaces

GAO Yun-Yan1, LI Hai-Xia1, OU Zhi-Ze1, HAO Ping1, LI Yi2, YANG Guo-Qiang3   

  1. 1. Ministry of Education Key Laboratory of Space, Applied Physics and Chemistry, School of Science, Northwestern Polytechnical University, Xi'an 710072, P. R. China;
    2. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
    3. Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China
  • Received:2011-04-10 Revised:2011-06-29 Published:2011-09-27
  • Contact: GAO Yun-Yan, OU Zhi-Ze E-mail:gaoyunyan@nwpu.edu.cn; ouzhize@nwpu.edu.cn
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (21073143), Chunhui Project from the Ministry of Education of China (Z2009-1-71002, Z2009-1-71006), and Northwestern Polytechnical University Foundation for Fundamental Research, China (JC200822, JC20100239).

摘要: 采用共沉淀法制备了Fe3O4磁性纳米粒子, 将其负载于氨基吡啶修饰多壁碳纳米管(MWCNT-AP)上, 得到具有良好的分散性和超顺磁性的Fe3O4/MWCNT-AP 复合物. 通过傅里叶变换红外(FT-IR)光谱、X射线衍射(XRD)和磁滞回线测量等方法对Fe3O4/MWCNT-AP 复合物进行了表征. 扫描电镜(TEM)结果表明: Fe3O4磁性纳米粒子多集中于碳纳米管MWCNT-AP 的端部, 形成的复合物在极性溶剂中具有良好的分散性和超顺磁性;辣根过氧化酶(HRP)可通过物理作用吸附于Fe3O4/MWCNT-AP 复合物表面. 酸性条件下(pH 4.0), Fe3O4/MWCNT-AP复合物使HRP的最大反应速率(vmax)提高了3倍.

关键词: 碳纳米管, 磁性纳米粒子, 过氧化酶, 纳米复合物, 超顺磁性

Abstract: Fe3O4 magnetic nanoparticles were prepared by co-precipitation and supported on the surface of aminopyridine-grafted multiwalled carbon nanotubes (MWCNT-AP), affording a superparamagnetic Fe3O4/MWCNT-AP composite with good dispersity. The Fe3O4/MWCNT-AP composites were characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and hysteresis curve measurements. Transmission electron microscopy (TEM) experimental results suggest that the Fe3O4 nanoparticles are mainly adsorbed on the tips of the MWCNT-AP. UV-Vis absorption and fluorescence results demonstrate that horseradish peroxidase (HRP) can be physically adsorbed onto the surfaces of the Fe3O4/MWCNT-AP composite. Under acidic conditions (pH 4.0), the maximum reaction rate (vmax) value of the HRP increases 3-fold after interaction with Fe3O4/MWCNT-AP.

Key words: Carbon nanotube, Magnetic nanoparticle, Peroxidase, Nanocomposite, Superparamagnetic property

MSC2000: 

  • O648