物理化学学报 >> 2012, Vol. 28 >> Issue (06): 1393-1397.doi: 10.3866/PKU.WHXB201204062

电化学和新能源 上一篇    下一篇

基于柔性衬底的ZnO葡萄糖酶电极制备及特性

李金华1,2, 李静1, 方铉1,2, 王晓华1, 魏志鹏1   

  1. 1. 长春理工大学理学院, 长春 130022;
    2. 长春理工大学国际纳米光子学与生物光子学联合研究中心, 长春 130022
  • 收稿日期:2012-03-09 修回日期:2012-04-06 发布日期:2012-05-17
  • 通讯作者: 李金华 E-mail:lijinhua2000@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(61006065, 61076039, 10804071), 高等学校博士学科点专项科研基金(20102216110001, 20112216120005), 吉林省科技发展计划(20091039, 20090555, 20101546, 20121816, 201201116)及吉林省教育厅项目(JYT2011-05, JYT2011-10, JYT2011-11)资助

Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate

LI Jin-Hua1,2, LI JING1, FANG Xuan1,2, WANG Xiao-Hua1, WEI Zhi-Peng1   

  1. 1. School of Science, Changchun University of Science and Technology, Changchun 130022, P. R. China;
    2. International Joint Research Center for Nanophotonics and Biophotonics, Changchun University of Science and Technology, Changchun 130022, P. R. China
  • Received:2012-03-09 Revised:2012-04-06 Published:2012-05-17
  • Contact: LI Jin-Hua E-mail:lijinhua2000@yahoo.com.cn
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (61006065, 61076039, 10804071), Research Fund for the Doctoral Program of Higher Education of China (20102216110001, 20112216120005), Developing Project of Science and Technology of Jilin Province, China (20091039, 20090555, 20101546, 20121816,201201116), and Foundation of Department of Education of Jilin Province, China (JYT2011-05, JYT2011-10, JYT2011-11).

摘要: 通过水热法在长有ZnO籽晶层的柔性聚酰亚胺(PI)衬底上生长了整齐的ZnO纳米棒, ZnO纳米棒的晶体结构和表面形貌通过X射线衍射(XRD)、扫描电子显微镜(SEM)等进行表征. 通过静电吸附方式, 将葡萄糖氧化酶(GOx)固定在其表面. 分别对GOx及修饰前后的ZnO纳米棒进行了紫外-可见光谱表征, 发现修饰后存在ZnO的吸收峰和GOx的特征吸收峰, 表明GOx固定在ZnO表面. 通过对修饰样品进行傅里叶变换红外(FTIR)光谱测试发现了与GOx相关的吸收峰, 这进一步表明GOx仍保持生物活性. 最后在循环伏安曲线的测试中, 这种在柔性衬底上制备的生物酶电极表现出非常灵敏的电流响应, 为制备柔性葡萄糖生物传感器奠定了实验基础.

关键词: 葡萄糖氧化酶, 柔性衬底, 生物传感器, 氧化锌

Abstract: Well-aligned ZnO nanorods (NRs) were grown on the ZnO seed layer of a polyimide (PI) coated flexible substrate using the hydrothermal method and used as a support matrix for the immobilization of glucose oxidase (GOx). The crystal structures and surface morphologies of the so-formed ZnO nanorods were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). GOx was immobilized on the surfaces of ZnO nanorods using electrostatic adsorption. Both the GOx and modified ZnO nanorods were characterized by UV-visible spectroscopy, and the absorption peaks of ZnO and GOx can be detected. Fourier transform infrared (FTIR) spectroscopy were used to characterize the chemical structure of the glucose oxide, which still maintained its biological activity. This study provides an experiment basis for the preparation of flexible glucose biosensors owing to the flexibility of the enzyme electrode, which showed a sensitive current response when tested by cyclic voltammetry.

Key words: Glucose oxidase, Flexible substrate, Biosensor, ZnO

MSC2000: 

  • O649