物理化学学报 >> 2012, Vol. 28 >> Issue (12): 2867-2873.doi: 10.3866/PKU.WHXB201209201

电化学和新能源 上一篇    下一篇

炭气凝胶纳米颗粒固定葡萄糖氧化酶的直接电化学

于志辉1, 宿婷婷1, 任翠华1, 李钒1, 夏定国2, 程水源1   

  1. 1 北京工业大学环境与能源工程学院, 北京 100124;
    2 北京大学工学院, 北京 100871
  • 收稿日期:2012-07-23 修回日期:2012-09-19 发布日期:2012-11-14
  • 通讯作者: 夏定国 E-mail:dgxia@pku.edu.cn
  • 基金资助:

    国家自然科学基金(50974006);北京市教委科研计划面上项目(KM201210005008);北京市人才强教深化计划-创新团队(PHR201007105)资助

Direct Electrochemistry and Immobilization of Glucose Oxidase on Nano-Carbon Aerogels

YU Zhi-Hui1, SU Ting-Ting1, REN Cui-Hua1, LI Fan1, XIA Ding-Guo2, CHENG Shui-Yuan1   

  1. 1 College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, P. R. China;
    2 College of Engineering, Peking University, Beijing 100871, P. R. China
  • Received:2012-07-23 Revised:2012-09-19 Published:2012-11-14
  • Contact: XIA Ding-Guo E-mail:dgxia@pku.edu.cn
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (50974006), General Program of Science and Technology Development Project of Beijing Municipal Education Commission of China (KM201210005008), Deepening Plan of Talented Personnel in Beijing-Promising Key Projects of China (PHR201007105).

摘要:

利用间苯二酚和甲醛在碱性环境下制备炭气凝胶(CA), 通过扫描电镜(SEM)、透射电镜(TEM)、比表面积测试Brunauer-Emmett-Teller (BET)等方法分析载体的形貌结构; 以CA为载体通过吸附法固定葡萄糖氧化酶(GOD)并修饰玻碳(GC)电极, 得到GOD/CA/GC电极. 在0.1 mol·L-1磷酸盐缓冲溶液中, 利用循环伏安法研究了GOD/CA/GC 电极的直接电化学行为和对葡萄糖的催化性能. 结果表明, 以CA为载体可以很好地固定GOD并保持其生物活性, 在无任何电子媒介体存在时, GOD在电极上实现了直接电子转移, GOD/CA/GC电极对葡萄糖具有很好的电催化性能.

关键词: 炭气凝胶, 葡萄糖氧化酶, 直接电化学

Abstract:

Nano-carbon aerogels (CA) were prepared by the reaction between resorcinol and formaldehyde in alkaline conditions. The morphology and microstructure of the CA was investigated by scanning electron microscopy and transmission electron microscopy. The specific area and the pore size distribution were calculated from the N2 adsorption-desorption isotherm. Glucose oxidase (GOD)/CA/ glassy carbon (GC) electrodes were prepared by immobilizing GOD on the surface of nano-carbon aerogels. The electrocatalytic properties of GOD/CA/GC electrodes were characterized by cyclic voltammetry in phosphate buffer medium (0.1 mol·L-1). The results showed that GOD can be immobilized on the nano-carbon aerogels, and that the biological activation of GOD was retained. The GOD/CA/GC electrode exhibits good direct electrochemical and electro-catalytic performance without electron mediators.

Key words: Carbon aerogel, Glucose oxidase, Direct electrochemistry

MSC2000: 

  • O646