物理化学学报 >> 2013, Vol. 29 >> Issue (07): 1588-1594.doi: 10.3866/PKU.WHXB201304233

生物物理化学 上一篇    下一篇

非支撑磷脂双层膜制备及温度对其力学性质的影响

王洋, 颜佳伟, 朱在稳, 赵雪芹, 钟赟鑫, 毛秉伟   

  1. 厦门大学化学化工学院化学系, 固体表面物理化学国家重点实验室, 福建 厦门 361005
  • 收稿日期:2013-01-31 修回日期:2012-04-22 发布日期:2013-06-14
  • 通讯作者: 颜佳伟 E-mail:jwyan@xmu.edu.cn
  • 基金资助:

    福建省自然科学基金(2012J01054); 国家自然科学基金(21021002)及中央高校基本科研业务费专项资金(2012121026)资助项目

Preparation of Freestanding Bilayer Lipid Membranes and the Effect of Temperature on Their Mechanical Properties

WANG Yang, YAN Jia-Wei, ZHU Zai-Wen, ZHAO Xue-Qin, ZHONG Yun-Xing, MAO Bing-Wei   

  1. State Key Laboratory of Physical Chemistry of the Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, P. R. China
  • Received:2013-01-31 Revised:2012-04-22 Published:2013-06-14
  • Supported by:

    The project was supported by the Natural Science Foundation of Fujian Province, China (2012J01054), National Natural Science Foundation of China (21021002), and Fundamental Research Funds for the Central Universities, China (2012121026).

摘要:

结合聚苯乙烯球刻蚀和微机电系统技术加工氮化硅纳米多孔膜, 并在其上用囊泡法制备非支撑磷脂双层膜, 通过温控原子力显微术(AFM)的成像模式和力曲线模式对非支撑磷脂双层膜的形貌和力学性质进行研究. 实验结果表明, 该方法制备的非支撑磷脂双层膜具有流动性, 能进行自我修复, 该特点有利于提供足够的非支撑磷脂双层膜区域用于其性质研究; 非支撑磷脂双层膜的膜破力和粘滞力均随着温度的升高而减小, 即膜的机械稳定性随着温度的升高而降低. 非支撑磷脂双层膜膜破力小于支撑磷脂双层膜的膜破力, 并且非支撑磷脂双层膜粘滞力随温度的变化趋势与支撑磷脂双层膜的变化趋势相反.

关键词: 原子力显微术, 非支撑磷脂双层膜, 氮化硅纳米多孔膜, 囊泡, 温度

Abstract:

Nanoporous silicon nitride membranes are fabricated by combining polystyrene colloidal lithography with Micro-Electro-Mechanical Systems, and then freestanding bilayer lipid membranes are constructed across the nanopores by the vesicle method. The topography and mechanical properties of the freestanding bilayer lipid membranes are investigated using the imaging and force curve modes of variable-temperature atomic force microscopy (AFM). The fluidity and self-repair of the freestanding bilayer lipid membranes are observed by AFM, which gives enough freestanding area for further investigations. Force curve measurements demonstrate that both breakthrough and adhesion forces decrease as the temperature increases; i.e., the mechanical stability of freestanding bilayer lipid membranes decreases as the temperature rises. The breakthrough force of freestanding bilayer lipid membranes is smaller than that of supported membranes. Moreover, the adhesion force of freestanding bilayer lipid membranes varies in the opposite manner to that of supported membranes.

Key words: Atomic force microscopy, Freestanding bilayer lipid membrane, Nanoporous silicon nitride membrane, Vesicle, Temperature

MSC2000: 

  • O647