溶菌酶蛋白在聚合物防污膜表面的吸附

doi:10.3866/PKU.WHXB201409021

物理化学学报 >> 2014, Vol. 30 >> Issue (11): 2149-2156.doi: 10.3866/PKU.WHXB201409021

溶菌酶蛋白在聚合物防污膜表面的吸附

胡立梅^1,2, 蔺存国², 王利², 苑世领¹

1. 山东大学胶体与界面化学教育部重点实验室, 济南 250100;
2. 海洋腐蚀与防护重点实验室, 中国船舶重工集团公司第七二五研究所, 山东青岛 266101

收稿日期:2014-07-31 修回日期:2014-09-02 发布日期:2014-10-30
通讯作者: 苑世领 E-mail:shilingyuan@sdu.edu.cn
基金资助:
国家重点基础研究发展规划项目(973) (2014CB643305)资助

Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface

HU Li-Mei^1,2, LIN Cun-Guo², WANG Li², YUAN Shi-Ling¹

1. Key Laboratory of Colloid and Interface Chemistry, Shandong University, Jinan 250100, P. R. China;
2. State Key Laboratory for Marine Corrosion and Protection, Luoyang Ship Material Research Institute LSMRI, Qingdao 266101, Shandong Province, P. R. China

Received:2014-07-31 Revised:2014-09-02 Published:2014-10-30
Contact: YUAN Shi-Ling E-mail:shilingyuan@sdu.edu.cn
Supported by:
The project was supported by the National Key Basic Research Program of China (973) (2014CB643305).

摘要/Abstract

摘要：

采用分子动力学模拟方法比较了溶菌酶蛋白在两种典型聚合物防污材料聚乙二醇(PEG)和聚二甲基硅氧烷(PDMS)表面的吸附行为, 在微观上探讨了聚合物膜表面性质对蛋白质吸附的影响. 根据蛋白质与聚合物膜之间的相互作用、能量变化及表面水化层分子的动力学行为, 解释了PEG防污涂层相对于PDMS表面具有更佳防污效果的原因: (1) 相比PDMS涂层, 蛋白质与PEG涂层的结合能量较低, 使其结合更加疏松; (2) 蛋白质吸附到材料表面要克服表面水化层分子引起的能障, PEG表面与水分子之间结合紧密, 结合水难于脱附, 造成蛋白质在其表面的吸附需要克服更高的能量, 不利于蛋白质的吸附.

关键词: 聚乙二醇, 聚二甲基硅氧烷, 防污材料, 分子动力学

Abstract:

Molecular dynamics simulations were used to compare the adsorption behavior of lysozyme on two typical antifouling polymer materials: poly(ethylene) glycol (PEG) and poly(dimethylsiloxane) (PDMS). The influence of the surface properties of the polymer films on protein adsorption is discussed at the microscale. Based on the interactions, energy changes between the protein and polymer films, and dynamical behavior of the hydration molecules near the polymer film, the reasons why the PEG antifouling coating has a better antifouling effect than the PDMS surface were determined as follows. (1) The lower binding energy between the protein and the PEG coating than between the protein and the PDMS coating makes the protein adsorb weaker on the PEG coating than on the PDMS coating. (2) The protein would adsorb on the film surface when overcoming the energy barrier caused by the hydration layer. Molecular water adsorbs on the PEG surface stronger than on the PDMS surface, and is difficult to desorb. Therefore, the protein needs to overcome a higher energy barrier to adsorb to the PEG surface than to the PDMS surface, and thus it is more difficult for protein to absorb on the PEG surface than on the PDMS surface.

Key words: Poly(ethylene) glycol, Poly(dimethylsiloxane), Antifouling material, Molecular dynamics

MSC2000:

O641

胡立梅, 蔺存国, 王利, 苑世领. 溶菌酶蛋白在聚合物防污膜表面的吸附[J]. 物理化学学报, 2014, 30(11): 2149-2156.

HU Li-Mei, LIN Cun-Guo, WANG Li, YUAN Shi-Ling. Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface[J]. Acta Phys. -Chim. Sin., 2014, 30(11): 2149-2156.

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溶菌酶蛋白在聚合物防污膜表面的吸附

Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface

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