物理化学学报 >> 2017, Vol. 33 >> Issue (4): 656-660.doi: 10.3866/PKU.WHXB201701162

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基于多肽和温敏聚合物的光交联囊泡的制备及表征

袁康,周雪,杜建忠*()   

  1. 1 同济大学材料科学与工程学院,高分子材料系,上海201804
    2 同济大学附属上海市第十人民医院,上海200072
  • 收稿日期:2016-12-12 发布日期:2017-03-23
  • 通讯作者: 杜建忠 E-mail:jzdu@tongji.edu.cn
  • 基金资助:
    国家自然科学基金(21374080);国家自然科学基金(21674081);国家自然科学基金(21611130175);上海市科委国际合作项目(15230724500);上海千人计划,中央高校基本科研业务费(0500219211);上海千人计划,中央高校基本科研业务费(1500219107)

Synthesis and Characterization of Thermo-Responsive Polypeptide-Based Vesicles with Photo-Cross-Linked Membranes

Kang YUAN,Xue ZHOU,Jian-Zhong DU*()   

  1. 1 Department of Polymeric Materials, School of Materials Science and Engineering, Tongji University, Shanghai 201804, P. R. China
    2 Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P. R. China
  • Received:2016-12-12 Published:2017-03-23
  • Contact: Jian-Zhong DU E-mail:jzdu@tongji.edu.cn
  • Supported by:
    the National Natural Science Foundation of China(21374080);the National Natural Science Foundation of China(21674081);the National Natural Science Foundation of China(21611130175);Shanghai International Scientific Collaboration Fund, China(15230724500);Shanghai 1000 Talents Plan, and Fundamental Research Funds for the Central Universities, China(0500219211);Shanghai 1000 Talents Plan, and Fundamental Research Funds for the Central Universities, China(1500219107)

摘要:

解决聚合物囊泡降解性和稳定性的矛盾是一个重要问题.本文通过可逆加成断裂链转移(RAFT)聚合和开环聚合(ROP)合成了一种聚[(N-异丙基丙烯酰胺-无规-7-(2-甲基丙烯酰氧基乙氧基)-4-甲基香豆素)-嵌段-(L-谷氨酸)] [P (NIPAM45-stat-CMA5)-b-PGA42]的两亲嵌段共聚物.囊泡膜由温敏性的聚N-异丙基丙烯酰胺(PNIPAM)和可光交联聚7-(2-甲基丙烯酰氧基乙氧基)-4-甲基香豆素(PCMA)组成.由囊泡膜向内外舒展的聚谷氨酸(PGA)链使囊泡稳定分散在水中,并且可进一步官能化.透射电子显微镜(TEM)和动态光散射(DLS)表征证实了囊泡的形貌和尺寸分布.本研究为制备基于多肽共聚物的可降解温敏囊泡提供了一个范例,并有望在纳米生物医药领域得到应用.

关键词: 囊泡, 多肽, 胶囊, 可逆加成断裂链转移聚合, N-羧基酸酐(NCA)

Abstract:

It is an important challenge to balance the degradability and stability of polymer vesicles. We report a thermo-responsive vesicle based on poly[(N-isopropyl acrylamide-stat-7-(2-methacryloyloxyethoxy)-4-methylcoumarin)-b-(L-glutamic acid)] [P (NIPAM45-stat-CMA5)-b-PGA42] diblock copolymer, which was synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization and ring-opening polymerization (ROP). The membrane of the vesicle consists of thermo-responsive PNIPAM and photo-cross-linkable PCMA. The PGA chains in the vesicle coronas can colloidally stabilize the vesicles in water and can be postfunctionalized for further applications. Transmission electron microscopy and dynamic light scattering studies confirmed the formation of vesicles. Overall, we prepared a new functional thermo-responsive vesicle based on polypeptide copolymers that may be used as nanocarriers for the facile loading of a range of molecules in future.

Key words: Vesicle, Peptide, Polymersome, Reversible addition fragmentation chain transfer, N-carboxyanhydride (NCA)

MSC2000: 

  • O648