物理化学学报 >> 2017, Vol. 33 >> Issue (5): 1051-1056.doi: 10.3866/PKU.WHXB201702201

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二氟沙星激发态氧化损伤氨基酸和脱氧鸟苷酸的激光光解研究

李海霞1,刘艳成2,唐睿智2,张鹏2,马六逵1,魏驰原1,王文锋2,*()   

  1. 1 南京工业大学浦江学院,南京211132
    2 中国科学院上海应用物理研究所,上海201800
  • 收稿日期:2016-11-18 发布日期:2017-04-20
  • 通讯作者: 王文锋 E-mail:wangwen@sinap.ac.cn
  • 基金资助:
    国家自然科学基金(21173252)

Reactions of Triplet-State Difloxacin with Amino Acids and dGMP: A Laser Flash Photolysis Study

Hai-Xia LI1,Yan-Cheng LIU2,Rui-Zhi TANG2,Peng ZHANG2,Liu-Kui MA1,Chi-Yuan WEI1,Wen-Feng WANG2,*()   

  1. 1 Pujiang Institute, Nanjing Tech University, Nanjing 211132, P. R. China
    2 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China
  • Received:2016-11-18 Published:2017-04-20
  • Contact: Wen-Feng WANG E-mail:wangwen@sinap.ac.cn
  • Supported by:
    The project was supported by the National Natural Science Foundation of China(21173252)

摘要:

本文利用激光光解法研究了二氟沙星激发态氧化损伤氨基酸和脱氧鸟苷酸等生物小分子。实验结果显示二氟沙星激发态水溶液氧化损伤色氨酸,酪氨酸,半胱氨酸和脱氧鸟苷酸的速率常数分别为1.97×108,1.48×108,1.72×108,6.92×107 dm3·mol-1·s-1。通过光谱分析和实验数据分析可以得出激发态二氟沙星氧化损伤色氨酸,酪氨酸,半胱氨酸和脱氧鸟苷酸是通过电子转移的方式进行的。

关键词: 激光光解, 二氟沙星, 激发态, 氨基酸

Abstract:

The reactions of triplet-state difloxacin (DFX) with various amino acids and deoxyguanylic acid in aqueous media were studied using laser flash photolysis. Tryptophan, tyrosine, cysteine, and 2'-deoxyguanosine-5'-monophosphate (dGMP) were found to completely quench the triplet state of DFX in aqueous solution, the corresponding second-order rate constants being 1.97×108, 1.48×108, 1.72×108, and 6.92×107 dm3·mol-1·s-1. The quenching mechanism involves electron transfer to the photoexcited triplet state of DFX from the tryptophan, tyrosine, cysteine, and dGMP moieties, followed by fast protonation of the resulting DFX anion radical.

Key words: Laser flash photolysis, Difloxacin, Triplet state, Amino acid

MSC2000: 

  • O644