物理化学学报 >> 2017, Vol. 33 >> Issue (8): 1573-1588.doi: 10.3866/PKU.WHXB201704284

所属专题: 高被引科学家特刊

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用于检测生物体系中线粒体活性氧的荧光探针

楼张蓉,李鹏,韩克利*()   

  • 收稿日期:2017-02-22 发布日期:2017-06-14
  • 通讯作者: 韩克利 E-mail:klhan@dicp.ac.cn
  • 作者简介:LOU Zhang-Rong, received her doctorate in Chemical Physics in 2014 from State Key Laboratory of Molecular Reaction Dynamics at the Dalian Institute of Chemical Physics (DICP). She is currently a research associate at DICP. Her research interests are focused on the design and synthesis of fluorescent probes for small biological molecules, as well as experimental and theoretical studies on excited-state dynamics of fluorescent probes|LI Peng, received his doctorate in organic chemistry in 2008 and subsequently became a research associate at DICP. He is currently an associate professor of the State Key Laboratory of Molecular Reaction Dynamics at DICP. His research interests are focused on the design and synthesis of reaction-based fluorescent probes for bioactive molecules|HAN Ke-Li, received his doctorate in 1990 from the State Key Laboratory of Molecular Reaction Dynamics at DICP and subsequently became a research associate. He pursued postdoctoral studies at Emory University and University of California at Davis in the years 1993 to 1995. In 1995, he became a full professor of Chemical Physics at DICP. Professor Han′s current research interests involve experimental and theoretical chemical dynamics
  • 基金资助:
    国家自然科学基金(21503224);国家自然科学基金(21673237)

Fluorescent Probes for Mitochondrial Reactive Oxygen Species in Biological Systems

Zhang-Rong LOU,Peng LI,Ke-Li HAN*()   

  • Received:2017-02-22 Published:2017-06-14
  • Contact: Ke-Li HAN E-mail:klhan@dicp.ac.cn
  • Supported by:
    The project was supported by the National Natural Science Foundation of China(21503224);The project was supported by the National Natural Science Foundation of China(21673237)

摘要:

线粒体不仅是细胞的能量单元,还是重要的活性氧产生场所。线粒体内的活性氧与正常的生理功能和人类疾病具有紧密的联系,包括细胞信号传导,损伤核酸蛋白质以及诱导氧化应激。然而,线粒体活性氧和细胞病态之间的复杂联系还研究得不够透彻。有效检测线粒体内活性氧的手段有助于研究线粒体内活性氧在各种人类疾病中所起的作用。近年来,发展了许多具有高灵敏度和选择性的荧光探针用于检测线粒体内活性氧。基于这一点,我们综述了用于选择性检测线粒体内活性氧的小分子荧光探针的研究进展,并详细讨论了荧光探针的设计、合成、特点及其应用。

关键词: 荧光探针, 线粒体, 活性氧, 小分子, 活细胞

Abstract:

In addition to being the energy powerhouse of the cell, mitochondria are an important source of reactive oxygen species (ROS) during the process of molecular oxygen metabolism. Mitochondrial ROS are closely associated with normal physiological functions as well as human diseases, and participate in cell signaling, nucleic acid and protein damage, and oxidative stress induction. However, the complicated interplay between mitochondrial ROS and the cellular pathological state has not been fully elucidated. It is expected that research on the mitochondrial ROS undertaking in the molecular pathogenesis of human diseases would benefit from development of efficient tools for the detection of these ROS. In recent years, an increasing number of fluorescent probes for mitochondrial ROS with high sensitivity and selectivity have been developed. Here, we present a review of the recent advances in small molecular fluorescent probes for selective detection of ROS inside the mitochondria. In this review, the design, synthesis, characteristics, and applications of the published fluorescent probes for mitochondrial ROS are discussed in detail.

Key words: Fluorescent probe, Mitochondria, Reactive oxygen species, Small molecule, Living cells

MSC2000: 

  • O641