物理化学学报 >> 2018, Vol. 34 >> Issue (5): 543-550.doi: 10.3866/PKU.WHXB201709291

论文 上一篇    

儿茶素-稀土配合物的合成、表征及抗菌活性

余康1,田翠翠1,李霞1,廖学品1,2,3,*(),石碧1,2,3   

  1. 1 四川大学生物质与皮革工程系,成都610065
    2 四川大学,皮革化学与工程教育部重点实验室,成都610065
    3 四川大学,制革清洁技术国家工程实验室,成都610065
  • 收稿日期:2017-07-31 发布日期:2018-01-24
  • 通讯作者: 廖学品 E-mail:xpliao@scu.edu.cn
  • 基金资助:
    国家重点基础材料技术提升与产业化项目

Synthesis, Characterization, and Antibacterial Activity of Rare Earth-Catechin Complexes

Kang YU1,Cuicui TIAN1,Xia LI1,Xuepin LIAO1,2,3,*(),Bi SHI1,2,3   

  1. 1 Department of Biomass and Leather Engineering, Sichuan University, Chengdu 610065, P. R. China
    2 Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, P. R. China
    3 National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, P. R. China
  • Received:2017-07-31 Published:2018-01-24
  • Contact: Xuepin LIAO E-mail:xpliao@scu.edu.cn
  • Supported by:
    the National Technology Improvement and Industrialization Project of Fundamental Materials, China

摘要:

以稀土(Re3+)和儿茶素(C)为原料,由液相合成法制备了3种(La3+-C、Gd3+-C、Er3+-C)配合物,结合傅里叶变换红外光谱、紫外光谱、X射线光电子能谱及配位数测定对配合物结构进行表征,确定了配合物的配位数为8。并采用牛津杯法、最小抑菌浓度(MIC)及最小杀菌浓度(MBC)等三种方法测定了Re3+-C对大肠杆菌、金黄色葡萄球菌、绿脓杆菌、沙门氏菌4种食源性细菌的抗菌性能。结果表明,这3种稀土配合物对各试验菌株均表现不同程度的抑制能力,相较Re3+和C而言,Re3+-C配合物的抑菌性能均有显著的提高。Re3+-C的抑菌活性顺序为:Gd3+-C > La3+-C > Er3+-C,其中Gd3+-C对4种细菌的MIC值分别为:1.550、0.097、0.780、1.550 μmol·mL−1,MBC值分别为3.100、0.194、1.550、1.550 μmol·mL−1,Gd3+-C对金黄色葡萄球菌表现出最佳的抑菌和杀菌能力。

关键词: 儿茶素, 稀土, 配合物, 食源性细菌, 抗菌活性

Abstract:

Three kinds of novel coordination compounds [Re3+-C] (Re = La, Gd, Er; C = Catechin) were synthesized by the liquid-phase method, and characterized by Fourier transform infrared (FT-IR) spectroscopy, ultraviolet-visible (UV) spectrophotometry, X-ray photoelectron spectroscopy (XPS), and coordination number determination. The results indicated that the coordination number of the complexes is 8. Moreover, the antibacterial activities of Re3+-C against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella were evaluated by the Oxford cup, minimum inhibition concentration (MIC), and minimum bactericidal concentration (MBC) approaches. Compared with Re3+ and C, the as-prepared complexes exhibited excellent antimicrobial activity toward the four strains. The MIC of Gd3+-C to these food-borne bacteria was 1.550, 0.0968, 0.775, and 1.550 μmol·mL−1, respectively, while the corresponding MBC values were 3.100, 0.194, 1.550, and 1.550 μmol·mL−1. It is clear that the Gd3+-C complex showed the best antibacterial and germicidal activity against S. aureus.

Key words: Catechin, Rare earth, Complex, Food-borne bacteria, Antibacterial activity

MSC2000: 

  • O641