物理化学学报 >> 1987, Vol. 3 >> Issue (02): 113-115.doi: 10.3866/PKU.WHXB19870201

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生物热动力学研究——大肠杆菌生长速率和激活能

汤厚宽; 谢昌礼; 宋昭华; 屈松生; 廖耀庭; 刘海水   

  1. 武汉大学化学系, 武汉; 广州军区第一陆军医院, 武汉
  • 收稿日期:1986-07-09 修回日期:1986-09-21 发布日期:1987-04-15

STUDIES ON BIOTHERMOKINETICS MEASUEREMENT OF MULTIPLICATION RATE CONSTANTS AND ACTIVATION ENERGY OF ESCHERICHIA COLI USING MICROCALORIMETER

Tang Houkuan; Xie Changli; Song Zhaohua; Qu Songsheng; Liao Yaoting; Liu Haishui   

  1. Department of Chemist; Wuhan University
    The First Army Hospital of Guangzhou Military Area
  • Received:1986-07-09 Revised:1986-09-21 Published:1987-04-15

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Abstract: In this paper, firstly, we have been studied the multiplication curves of E. coli at exponential phase of growth at 37 ℃ using microcalorimeter, LKB 2277 Bioactivity Monitor, and calculated the multiplication rate constant of E.coli using computer, which is k=0.0397±0.0025 min~(-1). Generation time, G=17.47 minutes, has been obtained from formula G=ln2/k, which is in close agreement with liter- ature value, G=17 minutes. Secondiy, using above method, rate constants of E. coli at different temperatures at exponential of growth have been determined. Finally, according to Arrhenius equation, the activation energy of E. coli multiplication has been obtained, which is 53.09 kJ mol~(-1).