物理化学学报 >> 1990, Vol. 6 >> Issue (04): 389-391.doi: 10.3866/PKU.WHXB19900402

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微量量热法研究螺旋藻活性物质对癌细胞和正常细胞的作用

张有民; 崔长海; 刘兆黔   

  1. 北京大学物理化学研究所;北京大学生物系
  • 收稿日期:1990-03-10 修回日期:1990-04-10 发布日期:1990-08-15
  • 通讯作者: 张有民

Stady of the Effect on the Growth of Tumor and Normal Cells with Bioactive Materials of Spirulina by Microcalorimetry Method

Zhang You-Min; Cui Chang-Hai; Liu Zhao-Qian   

  1. Institute of Physical Chemistry, Peking University, Beijing; Department of Biology, Peking University, Beijing
  • Received:1990-03-10 Revised:1990-04-10 Published:1990-08-15
  • Contact: Zhang You-Min

Abstract: The Ms 80 standard calvet microcalorimeter was used for measuring the thermo-grams of Hela, human breast carcinoma(Bcap-37) and diploid fibroblasts from human fetal lung (2BS) at 37 ℃ under the conditions of normal cell culture and culture in the presence of bioactive materials in Spirulina. There is a difference in the shapes of thermograms beteween tumor cells (Hela, Bcap-37) and normal coll (2BS) under the same condition. The former tended to ascent and the latter was almost parallel to abscissa during the measurements. The cell number was counted and the inhibit rate of growth and the death rate were obtained. The Sp. P was growth inhibitor and lethal to tumo cells, but had no influence on the growth of 2BS at the concentration of 100 μg·ml~(-1).