物理化学学报 >> 2001, Vol. 17 >> Issue (05): 477-480.doi: 10.3866/PKU.WHXB20010520

研究简报 上一篇    

贵金属离子非酶法生物还原机理初探

林种玉;傅锦坤;吴剑鸣;刘月英;程琥   

  1. 固体表面物理化学国家重点实验室,厦门大学化学系,1厦门大学生物系, 厦门 361005
  • 收稿日期:2000-10-24 修回日期:2001-01-11 发布日期:2001-05-15
  • 通讯作者: 吴剑鸣 E-mail:guozhong@xmu.edu.cn

Preliminary Study on the Mechanism of Nonenzymatic Bioreduction of Precious Metal Ions

Lin Zhong-Yu;Fu Jin-Kun;Wu Jian-Ming;Liu Yue-Ying;Cheng Hu   

  1. State Key Laboratory for Physical Chemistry of Solid Surface,Department of Chemistry,Department of Biology,Xiamen University,Xiamen 361005
  • Received:2000-10-24 Revised:2001-01-11 Published:2001-05-15
  • Contact: Wu Jian-Ming E-mail:guozhong@xmu.edu.cn

关键词: 巨大芽孢杆菌, 贵金属, 生物吸附, 非酶法生物还原, FTIR

Abstract: Preliminary study on the mechanism of nonenzymatic bioreduction of gold,silver,platinum,palladium and rhodium ions by Bacillus megatherium D01 biomass has been carried out by means of TEM,XRD,XPS and FTIR methods. The results showed that after contacted with D01 biomass,Au3+ has been completely reduced to elemental,cell bound Au0 for 2h and about 45% Rh3+ to Rh0 for 2 d at 30℃ and pH 3.5 without biochemical or chemical cofactors; the cardinal mechanism of D01 cell accumulating metal ions was biosorption,and the primary location where biosorption occurred was the D01 cell wall; active groups such as carbonyl of amido bond and carboxyl on the cell wall may complex or chelate with the precious metal ions; and aldose and ketose,i.e.,hydrolysate of part polysaccharide on the cell wall in acidic medium,served as the election donor,and reduced soluble precious metal ions to atoms.

Key words: Bacillus megatherium, Precious metal, Biosorpton, Non-enzymatic bioreduction-FTIR