物理化学学报 >> 2002, Vol. 18 >> Issue (07): 581-584.doi: 10.3866/PKU.WHXB20020702

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单晶硅作为新型的基因芯片基底的探索

孙豳;武巍;洪莲;赵新生   

  1. 北京大学化学与分子工程学院 分子动态与稳态结构国家重点实验室,化学生物学系,北京 100871
  • 收稿日期:2002-03-18 修回日期:2002-04-18 发布日期:2002-07-15
  • 通讯作者: 赵新生 E-mail:zhaoxs@chem.pku.edu.cn

Single Crystal Silicon Surface as a Novel Substrate of Gene-chip

Sun Bin;Wu Wei;Hong Lian;Zhao Xin-Sheng   

  1. State Key Laboratory of Molecular Dynamic and Stable Structures,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871
  • Received:2002-03-18 Revised:2002-04-18 Published:2002-07-15
  • Contact: Zhao Xin-Sheng E-mail:zhaoxs@chem.pku.edu.cn

摘要: 成功地将单晶Si(111)表面光化学引入官能团的技术与水溶性碳二亚胺催化多肽合成技术结合起来,制备并表征了ss-DNA 共价修饰的Si(111)表面.进一步证明了该表面具有特异性杂交的活性和反复使用的稳定性.为制备出可靠的、可反复使用的基因芯片提供了一种新的方法.

关键词: 基因芯片, 硅(111)表面, 寡聚核苷酸, 酯缩合反应, 共价键合, 反复使用

Abstract: Single strand oligodeoxynucleotide was covalently attached to the alkyl modified silicon (111) surface through ester condensation reaction and the result was characterized by fluorescent spectra.The wafers modified by ss-oligodeoxynucleotide were used as DNA probes and the reusability of the resultant wafer was investigated.Its perfect stability in target detection process may promote the wider and more efficient application of the gene chip technology.

Key words: Gene-chip, Silicon(111) surface, Oligodeoxynucleotide, Ester condensation reaction, Covalent immobilization, Reusability