物理化学学报 >> 2002, Vol. 18 >> Issue (12): 1076-1080.doi: 10.3866/PKU.WHXB20021205

研究论文 上一篇    下一篇

胆盐与磷酸钙的相互作用

俞彬;杨展澜;孙颖;翁诗甫;吴瑾光   

  1. 北京大学化学与分子工程学院,化学生物学系,稀土材料化学及应用国家重点实验室,北京 100871
  • 收稿日期:2002-03-12 修回日期:2002-06-10 发布日期:2002-12-15
  • 通讯作者: 杨展澜 E-mail:yzl@chem.pku.edu.cn

Interaction of Bile Salts with Calcium Phosphate

Yu Bin;Yang Zhan-Lan;Sun Ying;Weng Shi-Fu;Wu Jin-Guang   

  1. Department of Chemical Biology, State Key Laboratory of Rare Earth Material Chemistry and Application, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871
  • Received:2002-03-12 Revised:2002-06-10 Published:2002-12-15
  • Contact: Yang Zhan-Lan E-mail:yzl@chem.pku.edu.cn

摘要: 胆汁的pH条件下(pH=6~8),应该生成无定形磷酸钙(ACP),而在胆结石中磷酸钙通常以羟基磷灰石的形式出现.利用谱学方法研究了ACP与胆盐的作用.结果表明,胆盐以胶团的形式与ACP作用,在溶液中形成复合胶团,使其溶解度增加.不同类型胆盐与ACP的作用能力不同:脱氧胆酸钠(NaDC) > 牛磺胆酸钠(NaTC) > 胆酸钠(NaC).胆盐与ACP中结合钙的亲和能力大于结合钙的亲和能力,使ACP在胆汁的环境下容易转化为羟基磷灰石.

关键词: 胆结石, 胆盐, 磷酸钙, 红外光谱, 拉曼光谱

Abstract: Insoluble calcium salts are always observed in different types of gallstones, such as calcium phosphate, calcium carbonate and calcium bilirubinate. The major form of calcium phosphate in gallstone is calcium hydroxyapatite (HAP). Understanding of the transformation of amorphous calcium phosphate (ACP) into HAP in bile is necessary to prevent gallstones formation. The aim of this paper was to examine the interaction of bile salts, an important bio-surfactant, with ACP using vibrational spectroscopy. ACP [(Ca9-x(HPO4)2x(PO4)6-2x)],calcium hydrogen phosphate(CaHPO4•2H2O) and calcium phosphate(Ca3(PO4)2) precipitations were synthesized. CaHPO4 reacted with NaDC (0.1 mol•L-1) completely in solution, and mixed complex(NamCan(DC)2n+m) precipitate formed. In contrast, only part of Ca3(PO4)2 reacted with NaDC, and the aggregate of NaDC in solution increased by Ca2+bridge. It indicated that the micelle of NaDC bound to Ca2+ on the solid surface of both CaHPO4 and Ca3(PO4)2, and the reactivity of CaHPO4 was more stronger than that of Ca3(PO4)2. ACP reacted with NaDC, NaTC and NaC respectively. The results demonstrated that the solubility of ACP increased with increase of bile salts content in solution. The reacting capacity of bile salts for ACP has the following order: NaDC >NaTC >NaC. The affinity of bile salts micelle to calcium binding to in ACP is stronger than binding to PO43-. Therefore, ACP transfers easily into HAP under bile condition.

Key words: Gallstone, Bile salts, Calcium phosphate, FT-IR, FT-Raman