物理化学学报 >> 2013, Vol. 29 >> Issue (05): 1028-1034.doi: 10.3866/PKU.WHXB201302271

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HA/PDM自组装复合胶体粒子及其乳化性能

王娟勤, 白绘宇, 易成林, 刘娜, 刘晓亚   

  1. 江南大学化学与材料工程学院, 食品胶体与生物技术教育部重点实验室, 江苏 无锡 214122
  • 收稿日期:2012-11-21 修回日期:2013-02-26 发布日期:2013-04-24
  • 通讯作者: 刘晓亚 E-mail:lxyjiangna@126.com
  • 基金资助:

    国家自然科学基金(20974041)资助项目

Self-Assembled HA/PDM Particles and Emulsification Properties

WANG Juan-Qin, BAI Hui-Yu, YI Cheng-Lin, LIU Na, LIU Xiao-Ya   

  1. Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu Province, P. R. China
  • Received:2012-11-21 Revised:2013-02-26 Published:2013-04-24
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (20974041).

摘要:

以阴离子天然大分子透明质酸(HA)和阳离子单体甲基丙烯酸二甲氨基乙酯(DM)组成带相反电荷的聚合物/单体复合体系, DM通过水相原位聚合可制备荷正电的聚甲基丙烯酸二甲氨基乙酯(PDM), PDM与HA间的静电作用可诱导两者在水溶液中进行自组装, 得到HA/PDM复合胶体粒子. 用傅里叶变换红外(FTIR)光谱仪对HA/PDM复合物结构进行了表征. 用动态激光光散射(DLS)研究了HA与PDM复合体系在水溶液中的自组装行为, 并表征了反应时间对HA/PDM复合胶体粒子粒径的影响. 利用透射电镜(TEM)表征了胶体粒子的形貌. 考察了溶液pH 对胶体粒子粒径及zeta 电位的影响, 并对胶体粒子的乳化性能进行初步探索. 结果表明:DM单体聚合前, 无HA/DM复合物聚集体形成; 而随着DM的逐步聚合, HA与PDM可通过静电作用逐渐组装形成球状HA/PDM复合胶体粒子, 其粒径随反应时间延长逐渐减小并趋于稳定. 同时, 该复合胶体粒子具有pH敏感性和乳化性, 乳化性能较纯HA和PDM有较大提高.

关键词: 透明质酸, 聚甲基丙烯酸二甲氨基乙酯, 静电作用, 自组装, 胶体粒子, 乳化

Abstract:

The anionic biomacromolecule, hyaluronic acid (HA), and cationic monomer, 2-(dimethylamino) ethyl methacrylate (DM), were used to construct an opposing charge polymer/monomer complex. Positively charged poly[2-(dimethylamino)ethyl methacrylate] (PDM) was prepared when DM monomers polymerized in aqueous solution. Self-assembly between PDM and HA was driven by electrostatic interaction, and HA/PDM complex colloid particles were formed in the aqueous medium. The HA/PDM complex structure was characterized by Fourier transform-infrared (FTIR) spectroscopy. The self-assembly behavior between HA and PDM, and the influence of polymerization time on HA/PDM complex colloid particle size, were investigated by dynamic laser scattering (DLS). Morphologies of the colloid particles were characterized by transmission electron microscopy (TEM). The influence of pH on the size and zeta potential of the colloid particles was investigated, and the emulsification of the colloid particles was preliminarily explored. The results showed that no HA/DM complex aggregates were formed before the polymerization of DM monomers. Spherical HA/PDM particles were spontaneously formed through electrostatic interaction between HA and PDM, coinciding with the gradual polymerization of DM. Particle sizes gradually decreased and stabilized with increasing polymerization time. The HA/PDM particles were pH sensitive and possessed improved emulsification properties compared with uncomplexed HA and PDM.

Key words: Hyaluronic acid, Poly[2-(dimethylamino)ethyl methacrylate], Electrostatic interaction, Self-assembly, Colloid particle, Emulsification

MSC2000: 

  • O648