基于微电极阵列的睡眠剥夺大鼠海马区神经电活动检测

doi:10.3866/PKU.WHXB201907033

物理化学学报 >> 2020, Vol. 36 >> Issue (12): 1907033.doi: 10.3866/PKU.WHXB201907033

所属专题：神经界面

基于微电极阵列的睡眠剥夺大鼠海马区神经电活动检测

陆泽营¹^,², 徐声伟¹, 王昊¹^,², 刘军涛¹, 高飞¹^,², 宋轶琳¹, 谢精玉¹^,², 肖桂花¹^,², 张禹¹^,², 戴玉川¹^,², 王云¹^,², 曲丽娜³^,*(), 蔡新霞¹^,²^,*()

¹ 中国科学院电子学研究所，传感技术国家重点实验室，北京 100190
² 中国科学院大学，北京 100049
³ 中国航天员科研训练中心，航天医学基础与应用国家重点实验室，北京 100094

收稿日期:2019-07-10 录用日期:2019-09-11 发布日期:2019-09-16
通讯作者: 曲丽娜,蔡新霞 E-mail:linaqu@263.net;xxcai@mail.ie.ac.cn
基金资助:
国家重大研究发展计划项目(2017YFA0205902);国家自然科学基金(61527815);国家自然科学基金(61771452);国家自然科学基金(61775216);中国科学院前沿科学重点研究项目(QYZDJ-SSW-SYS015)

Detection of Neuronal Activity in the Hippocampus of Sleep Deprived Rats Using Microelectrode Arrays

Zeying Lu¹^,², Shengwei Xu¹, Hao Wang¹^,², Juntao Liu¹, Fei Gao¹^,², Yilin Song¹, Jingyu Xie¹^,², Guihua Xiao¹^,², Yu Zhang¹^,², Yuchuan Dai¹^,², Yun Wang¹^,², Lina Qu³^,*(), Xinxia Cai¹^,²^,*()

¹ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China
² University of Chinese Academy of Sciences, Beijing 100049, P. R. China
³ State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing 100094, P. R. China

Received:2019-07-10 Accepted:2019-09-11 Published:2019-09-16
Contact: Lina Qu,Xinxia Cai E-mail:linaqu@263.net;xxcai@mail.ie.ac.cn
Supported by:
the National Key Research and Development Program of China(2017YFA0205902);the National Natural Science Foundation of China(61527815);the National Natural Science Foundation of China(61771452);the National Natural Science Foundation of China(61775216);the Key Research Programs of Frontier Sciences, CAS(QYZDJ-SSW-SYS015)

摘要/Abstract

摘要：

睡眠剥夺是一种能够实现个体睡眠部分或完全丧失的技术手段，由睡眠干扰所引发的逐渐积累的睡眠压力能导致多种生理方面的变化，甚至是个体的死亡。在本次研究中，我们使用旋转滚动式睡眠剥夺仪对大鼠进行了长达14天的睡眠剥夺，同时我们制作了一种16通道的微电极阵列并将其植入到大鼠海马区进行实时电生理信号检测。结果显示睡眠剥夺之后，大鼠海马区内的椎体神经元和中间神经元动作电位幅值提升，两种神经元动作电位的发放频率也显著增大。同时，场电位的波动也更加剧烈。神经细胞在睡眠剥夺后的快速发放模式表明长期清醒状态下神经细胞兴奋性的提升。此外，场电位在0–50 Hz频段的平均功率计算结果显示，睡眠剥夺之后各个频段的功率均有所提升，且在δ频段的变化最为明显。场电位在低频段的功率改变表明了睡眠剥夺所致的睡眠压力增大，此改变还将会进一步损伤大脑的相关功能。

关键词: 睡眠剥夺, 神经细胞活动, 微电极阵列, 动作电位, 场电位

Abstract:

Sleep deprivation (SD) is the partial or complete loss of sleep and has long been used as a tool in sleep research to interfere with normal sleep cycles in rodents and humans. The progressively-accumulating sleep pressure induced by sleep deprivation can lead to a variety of physiological changes and even death. Compared to traditional detection methods, in vivo detection of neuronal activity using micro-electromechanical system (MEMS) technology following sleep deprivation can help fully elucidate the effects of sleep deprivation at the cellular level. Herein, a computer-controlled rotary roller was used to completely deprive rats of sleep for 14 days and 16-channel microelectrode arrays (MEAs) were fabricated and implanted into the rat hippocampus to measure neural spikes and local field potentials (LFPs) in real-time. The hippocampus is involved in learning and memory and has been the focus of intensive research aimed at understanding the function of sleep. This study was performed to measure the changes in neuronal activity in the rat hippocampus induced by sleep deprivation as well as their overall impact on the brain. After sleep deprivation, both the pyramidal- and inter-neurons showed a higher amplitude and more intense firing patterns. The fast-firing pattern of the neurons after sleep deprivation indicated elevated excitability in the prolonged awake state. In addition, the LFP of the sleep deprived rats fluctuated more frequently. The power of the LFPs in the low-frequency band (0–50 Hz) was calculated, showing increased power of the delta, theta, alpha, and beta bands after sleep deprivation, especially for the delta band (0.1–4 Hz). Generally, LFPs are generated by all types of neural activity in the neural circuit, and the changes in the low frequency band power suggested decreased arousal and increased sleep pressure induced by sleep deprivation, which could further impair brain function. This study was mainly aimed at measuring electrophysiological changes induced by sleep deprivation in the rat brain. Typically, neuronal activity changes were accompanied by the alternation of specific neurotransmitters in the brain. In the future, it will be essential to focus on measuring the concurrent change of electrophysiological and neurochemical signals to better examine the impact of sleep deprivation on brain function.

Key words: Sleep deprivation, Neuronal activity, Microelectrode array, Spike, Local field potential

MSC2000:

O646

陆泽营, 徐声伟, 王昊, 刘军涛, 高飞, 宋轶琳, 谢精玉, 肖桂花, 张禹, 戴玉川, 王云, 曲丽娜, 蔡新霞. 基于微电极阵列的睡眠剥夺大鼠海马区神经电活动检测[J]. 物理化学学报, 2020, 36(12): 1907033.

Zeying Lu, Shengwei Xu, Hao Wang, Juntao Liu, Fei Gao, Yilin Song, Jingyu Xie, Guihua Xiao, Yu Zhang, Yuchuan Dai, Yun Wang, Lina Qu, Xinxia Cai. Detection of Neuronal Activity in the Hippocampus of Sleep Deprived Rats Using Microelectrode Arrays[J]. Acta Phys. -Chim. Sin., 2020, 36(12): 1907033.

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	Power/mW (Normal)	Power/mW (SD)
Delta (0–4 Hz)	1.492 ± 0.061	4.681 ± 0.680^**
Theta (4–8 Hz)	0.959 ± 0.028	1.459 ± 0.132^*
Alpha (8–13 Hz)	1.036 ± 0.034	1.939 ± 0.170^**
Beta (13–30 Hz)	0.956 ± 0.043	2.454 ± 0.199^**

基于微电极阵列的睡眠剥夺大鼠海马区神经电活动检测

Detection of Neuronal Activity in the Hippocampus of Sleep Deprived Rats Using Microelectrode Arrays

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