物理化学学报 >> 2007, Vol. 23 >> Issue (12): 1827-1830.doi: 10.1016/S1872-1508(07)60085-0

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微流路中利用DNA选择性固定蛋白质

郭素; 薛面起; 钱民协; 曹廷炳; 赵新生   

  1. 北京大学化学与分子工程学院化学生物学系, 分子动态与稳态结构国家重点实验室, 北京分子科学国家实验室, 北京 100871; 中国人民大学化学系, 北京 100872
  • 收稿日期:2007-08-22 修回日期:2007-10-08 发布日期:2007-11-30
  • 通讯作者: 赵新生 E-mail:zhaoxs@pku.edu.cn

Selective Protein Immobilization via DNA Conjugation under Microfluidic Laminar Flow

GUO Su; XUE Mian-Qi; QIAN Min-Xie; CAO Ting-Bing; ZHAO Xin-Sheng   

  1. Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China; Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China
  • Received:2007-08-22 Revised:2007-10-08 Published:2007-11-30
  • Contact: ZHAO Xin-Sheng E-mail:zhaoxs@pku.edu.cn

摘要:

报道了一种可控的通过DNA复合物在微流路中杂交固定蛋白质的方法. 微流路系统中的玻璃基底上固定寡聚核苷酸, 其中的层流提供了不同的DNA-蛋白质复合物. DNA的特异性识别可以将蛋白通过表面寻址固定在基底上. 并且在体系中引入了全内反射荧光技术来追踪整个过程. 此方法的特异性和灵敏度均较高, 且蛋白质的固定和去除可重复. 实验结果显示, 同时检测特异性和非特异性的识别, 可以有效提高生物检测的准确性. 这项技术可以提高具有微流路结构的生物传感器装置的检测质量.

关键词: 微流路, 蛋白质传感器, DNA引导的固定, 全内反射荧光

Abstract:

The feasibility of controlled protein immobilization via DNA conjugation by utilizing laminar flow in a microfluidic device was demonstrated. The glass surface in a microchannel was treated by oligonucleotides. The laminar flow brought different protein-DNA conjugates parallel into the microchannel. DNA recognition allows proteins to be delivered to the desired location. The total internal reflection fluorescence was also applied to monitor the process. Both the specificity and sensitivity were high, and the immobilization and removal of the proteins were repeatable. It was shown that with parallel detection of specific and non-specific recognitions, the accuracy of bio-assay would be effectively enhanced. This strategy could improve the performing quality of biosensors in microfluidic devices.

Key words: Microfluidic device, Protein sensor, DNA-directed immobilization, Total internal reflection fluorescence

MSC2000: 

  • O647