物理化学学报 >> 2006, Vol. 22 >> Issue (07): 868-872.doi: 10.3866/PKU.WHXB20060720

研究简报 上一篇    下一篇

固定化辣根过氧化物酶在有机/水混合溶液中的电化学

刘慧宏;万永清;陈显堂;邹国林   

  1. 襄樊学院化学与生物科学系, 湖北 襄樊 441053; 武汉大学生命科学学院, 武汉 430072
  • 收稿日期:2006-01-16 修回日期:2006-03-05 发布日期:2006-06-27
  • 通讯作者: 刘慧宏 E-mail:liuhh@xfu.edu.cn

Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture

LIU Hui-Hong;WAN Yong-Qing;CHEN Xian-Tang;ZOU Guo-Lin   

  1. Department of Chemistry & Biology, Xiangfan University, Xiangfan 441053, P. R. China; College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China
  • Received:2006-01-16 Revised:2006-03-05 Published:2006-06-27
  • Contact: LIU Hui-Hong E-mail:liuhh@xfu.edu.cn

摘要: 用魔芋多糖(KGM)将辣根过氧化物酶(HRP)固定在玻碳电极(GCE)表面, 制备了HRP-KGM膜修饰电极. 在乙醇等亲水性有机溶剂与水的混合溶液中, 包埋在KGM中的HRP 可以与电极发生直接电子传递, 且能催化还原过氧化氢、氢过氧化异丙基苯、氢过氧化叔丁基、过氧化丁酮等过氧化物. HRP-KGM膜修饰电极具有较好的稳定性和重现性, 可用于这些物质的定量检测.

关键词: 辣根过氧化物酶, 电化学, 过氧化物

Abstract: Horseradish peroxidase (HRP) was immobilized on glassy carbon electrode (GCE) surface by konjac glucomannan (KGM). HRP entrapped in the KGM film underwent fast direct transfer-electron reactions in water-organic solvent mixtures. The electrocatalysis of peroxides, such as hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, and 2-butanone peroxide, by the immobilized HRP was also explored. Stability and reproducibility of the modified electrode made it possible to determine the organic peroxides.

Key words: Horseradish peroxidase, Electrochemistry, Peroxide