物理化学学报 >> 2001, Vol. 17 >> Issue (01): 32-36.doi: 10.3866/PKU.WHXB20010107

研究论文 上一篇    下一篇

掺杂-涂膜聚苯胺尿酸酶电极的生物电化学特性

阚锦晴;侯军花;穆绍林   

  1. 扬州大学理学院化学化工系,扬州 225002
  • 收稿日期:2000-05-08 修回日期:2000-07-03 发布日期:2001-01-15
  • 通讯作者: 阚锦晴 E-mail:zatan@yzu.edu.cn

Bioelectrochemical Characters of Doping-coating Polyanilineuricase Electrode

Kan Jin-Qing;Hou Jun-Hua;Mu Shao-Lin   

  1. Department of Chemistry,School of Sciences,Yangzhou University,Yangzhou 225002
  • Received:2000-05-08 Revised:2000-07-03 Published:2001-01-15
  • Contact: Kan Jin-Qing E-mail:zatan@yzu.edu.cn

摘要: 用聚苯乙烯石蜡体系制备涂膜聚苯胺尿酸酶电极的方法简单,在 0.35 V(vs SCE)时的响应时间仅为 30 s 左右.涂膜聚苯胺尿酸酶电极酶催化反应的最适 pH 和电势依赖性与聚苯胺尿酸酶电极酶催化反应的最适 pH 和电势依赖性相似,但涂膜聚苯胺尿酸酶电极酶催化反应的活化能(19.9 kJ•mol-1)较无膜聚苯胺尿酸酶电极酶催化反应的活化能(29.9 kJ•mol-1)低,而前者较后者的稳定性好(前者超过180天).

关键词: 涂抹掺杂技术, 聚苯乙烯, 生物电化学, 聚苯胺, 尿酸酶

Abstract: A simple dopingcoating technique is described for immobilizing uricase using polyaniline and polystyrene and paraffin.The polyaniline uricase electrode with polystyrene coating has a fast response time (about 30 s at 0.35 V vs.SCE).The optimum pH and the dependence on potential are similar to those of polyaniline uricase electrode,the activation energy and stability of the polyaniline uricase electrode with polystyrene coating are 19.9 kJ•mol-1 and over 180 days,respectively.

Key words: Doping-coating method, Polystyrene, Bioelectrochemistry, Polyaniline,  Uricase