物理化学学报 >> 2000, Vol. 16 >> Issue (12): 1073-1079.doi: 10.3866/PKU.WHXB20001204

研究论文 上一篇    下一篇

肌动蛋白与顺铂、反铂的相互作用

杨勇, 段丽丽, 李芝芬, 曾慧慧, 王保怀, 张有民   

  1. 北京大学物理化学研究所 100871|北京医科大学药学院 北京 100083
  • 收稿日期:2000-03-15 修回日期:2000-06-05 发布日期:2000-12-15
  • 通讯作者: 李芝芬 E-mail:lyjzf@pku.edu.cn

Interaction between Actin and Cisplatin or Transplatin

Yang Yong, Duan Li-Li, Li Zhi-Fen, Zeng Hui-Hui, Wang Bao-Huai, Zhang You-Min   

  1. Institute of Physical Chemistry,Peking University 100871|School of Pharmaceutical Science,Beijing Medical University,Beijing 100083
  • Received:2000-03-15 Revised:2000-06-05 Published:2000-12-15
  • Contact: Li Zhi-Fen E-mail:lyjzf@pku.edu.cn

摘要:

利用1H-NMR研究了肌动蛋白(actin)与顺铂、反铂的相互作用,测定了肌动蛋白的聚合临界浓度,并研究了顺伯、反铂对它的影响。实验结果显示顺伯和反驳都能导致微丝(F-actin)解聚。在相同浓度下,反铂的作用更加明显。对于肌动蛋白单体(G-actin),顺铂在低浓度下促进其聚合。反铂则只表现出抑制G-actin的聚合。讨论了顺铂、反铂与G-actin可能的结合位点,顺铂可能与Cys-10和Cys-285结合,而反铂不与Cys-10结合。

关键词: 肌动蛋白, 顺铂, 反铂, 1H-NMR

Abstract:

The interaction between actin and cisplatin or transplatin hes been studied by using the 1H-NMR spectra and measuring the critical concentration of actin polymerization.These studies show that both cisplatin and transplatin induce a depolymerization of F-actin,and that the effect of transplatin is more obvious than that of cisplatin at the same concentration.It is found that cisplatin promotes a polymerization of G-actin at low concentration and inhibits it at high concentration.while transplatin inhibits the polymerization at any concentration.The binding sites of cisplatin/transplatin to G-actin are discussen.It is probable that cisplatin binds with Cys-10 and Cys-285,while tracsplatin does not bind with Cys-10.

Key words: Actin, Cisplatin, Transplatin, 1H-NMR